Role of nitric oxide in biological effects of vascular endothelial growth factor

作     者：Qigong LIU Yan ZENG Jiani LIU Shan YE Yongdong LI Zaiying LU 

作者机构：Department of Internal CardiologyTongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China 

出 版 物：《Frontiers of Medicine》 (医学前沿（英文版）)

年 卷 期：2009年第3卷第3期

页      面：284-286页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by a grant from the Natural Science Foundation of Hubei Province(No.2003ABA135) 

主　　题：vascular endothelial growth factor nitric oxide N-nitro-L-arginine methyl ester vascular endothelial cells 

摘      要：To evaluate the role of nitric oxide in the biological effects of vascular endothelial growth factor(VEGF)and the possible mechanism of VEGF,the in vitro cultured vascular endothelial cells of rabbit aorta were divided into control group,VEGF-treated group and VEGF+N-nitro-L-arginine methyl ester(L-NAME)-treated *** absorbance(A)value of vascular endothelial cells and the levels of prostaglandin(PGI2),endothelin-1(ET-1)and von Willebrand factor(vWF)in the supernatant were observed by water-soluble tetrazo-lium salt assay,radioimmunoassay and enzyme-linked immunosorbent *** A values and PGI2 in VEGF-treated group and VEGF+L-NAME-treated group were higher than those in control group(P0.05 and P0.01).The ET-1 and vWF were significantly decreased in VEGF-treated group and VEGF+L-NAME-treated group com-pared with the control(P0.05 and P0.01).These results indicate that VEGF could promote the proliferation and secretion of PGI2 and inhibit the secretion of ET-1 and vWF in vascular endothelial cells and that L-NAME could inhibit the effect of VEGF *** oxide is an important mediator in the process of stimulating prolifera-tion and regulating secretion of vascular endothelial cells by VEGF.