Mass spectrometry based proteomics,background,status and future needs

作     者：Peter Roepstorff 

作者机构：Department of Biochemistry and Molecular BiologyUniversity of Southern Denmark Campusvej 55DK 5230 Odense MDenmark 

出 版 物：《Protein & Cell》 (蛋白质与细胞（英文版）)

年 卷 期：2012年第3卷第9期

页      面：641-647页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

主　　题：proteomics 2D-PAGE LC-MS isoforms phosphorylation 

摘      要：An overview of the background for proteomics and a description of the present state of art are given with a description of the main strategies in *** advantages and limitations of the two major strategies,2D-gel based and LC-MS based,are discussed and a combination for the two,CeLC-MS is described.A number of challenging problems which have been solved using different proteomics strategies including the advantage of organell enrichment or modifications specific peptide isolation to get deeper into the proteome are *** the present status and future needs discussed.