Cloning,sequencing and prokaryotic expression of cDNAs for antifreeze protein family from Beetle Tenebrio molitor
Cloning,sequencing and prokaryotic expression of cDNAs for antifreeze protein family from Beetle Tenebrio molitor作者机构：College of Life Science and TechnologyXinjiang UniversityXinjiang Key Laboratory of Biological Resources and Genetic EngineeringUrumqi 830046China
出 版 物：《Frontiers in Biology》 (生物学前沿（英文版）)
年 卷 期：2008年第3卷第3期
页 面：279-286页
核心收录：
学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 0713[理学-生态学]
主 题：Tenebrio molitor antifreeze proteins cDNA fragment sequence analysis prokaryotic expression
摘 要：Partial cDNA sequences coding for antifreeze proteins in Tenebrio molitor were obtained by *** analysis revealed nine putative cDNAs with a high degree of homology to Tenebrio molitor antifreeze protein genes published in *** recombinant pGEX-4T-1-tmafp-XJ430 was introduced into *** BL21 to induce a GST fusion protein by ***-PAGE analysis for the fusion protein shows a band of 38 ***3-tmafp-XJ430 was injected into mice to generate antiserum which was later detected by indirect *** titer of the antibody was 1:*** blotting analysis shows that the antiserum was specifically against the antifreeze *** results laid the foundation for further studies on the properties and functions of insect antifreeze proteins.
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