Enhancement of radiosensitivity of lung adenocarcinoma using a decoction from the Fuzhengzengxiao formula

作     者：Zhang Qiaoli Wu Tong Lei Yong Li Bo Liu Weiyi Tian Yehong Zhao Weipeng Huang Jingchang 

作者机构：Department of Traditional Chinese MedicineBeijing Hepingli Hospital Department of Traditional Chinese MedicineBeijing Ditan HospitalCapital Medical University Graduate School of Beijing University of Traditional Chinese Medicine Health Science CenterPeking University Department of Acupuncture and Minimally Invasive OncologyBeijing University of Chinese Medicine Third Affiliated Hospital 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2015年第35卷第6期

页      面：690-696页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by a Grant from the National Natural Science Foundation of China:the Effect of TCM Combined Radiotherapy and RNAi Suppression on the Protein Expression Changes of S100A9 & Cyclophilin A and Radiosensitivity in Lung Adenocarcinoma(No.81072925) 

主　　题：Calgranulin B RNA interference Radia-tion tolerance Adenocarcinoma of lung Fuzhengz-engxiao formula 

摘      要：OBJECTIVE: To study the effects of a decoction of Fuzhengzengxiao formula on lung adenocarcinoma regarding the inflammatory protein S100A9 known to enhance cancer cell ***: A nude mouse model of human lung adenocarcinoma was established. The mice were randomly divided into four groups using the random number table method: Group Ⅰ, control;Group Ⅱ, treatment with a decoction of the Fuzhengzengxiao formula alone; Group Ⅲ, treatment with radiotherapy alone; and Group Ⅳ, treatment with radiotherapy plus a decoction of Fuzhengzengxiao formula. When the tumor body was 1 cm^3 in diameter, the tumor bearing mice in GroupsⅢ and Ⅳ were irradiated at a single dose of 10 Gy and the tumor inhibition rate was *** expression of S100A9 was determined using Western blotting and q-PCR(Real-time Quantitative PCR Detecting System). The sensitivity of cells containing RNAi S100A9 to radiotherapy was evaluated using the Click multiple target model,and the cell cycle was analyzed using flow ***: Relative to the control group,the expression of S100A9 in the tumors in each treatment group was decreased,especially in Group Ⅳ. The sensitizing enhancement ratio(SER) Dq was 1 after RNAi S100A9; it decreased the surviving fraction after a 2 Gy dose exposure,and also the D_0 and Dq of the tumor cells; in addition, the radiosensitivity of G_2/M cells was significantly ***: The decoction of the Fuzhengzengxiao formula downregulated the expression of S100A9 in lung adenocarcinoma cells.