Effect of hematopoietic stem cell on tumor necrosis factor-αexpression, spiral artery remodeling and placental apoptosis in lead-exposed pregnant mice

作     者：Widjiati Suryo Kuncorojakti Hendy Hendarto Viski Fitri Hendrawan Aulanni'am Aulanni'am 

作者机构：Faculty of Veterinary MedicineUniversitas AirlanggaSurabayaIndonesia Faculty of MedicineUniversitas AirlanggaSurabayaIndonesia Faculty of Veterinary MedicineUniversitas BrawijayaMalangIndonesia 

出 版 物：《Asian pacific Journal of Reproduction》 (亚太生殖杂志（英文版）)

年 卷 期：2017年第6卷第4期

页      面：158-163页

学科分类：0710[理学-生物学] 1002[医学-临床医学] 0905[农学-畜牧学] 0906[农学-兽医学] 0901[农学-作物学] 100214[医学-肿瘤学] 0902[农学-园艺学] 10[医学] 

基　　金：Universitas Airlangga  UNAIR  (RKAT 2014) 

主　　题：Pregnancy Hematopoietic stem cell Spiral artery remodeling Apoptosis 

摘      要：Objective:To evaluate the effectiveness of hematopoietic stem cell (HSC) on TNF-αexpression, spiral artery remodeling, and placental ***: The study used pregnant mice divided to five groups of treatment: Group 1, without treatment;Group 2, 1/12 LD50 of lead and Aquadest;Group 3, 1/16 LD50 of lead and Aquadest, Group 4, 1/12 LD50 of lead and 1×107 HSC;and group 5, 1/16 LD50 of lead and 1×107 HSC. Histopathology examination was performed to identify spiral artery remodeling. Immunohistochemistry analysis was conducted to characterize TNF-α expression and the apoptosis index. The data from all five groups were compared using the ANOVA ***:TNF-α expression differed significantly among the groups that were not exposed to lead, exposed to lead and exposed to lead after HSC treatment (P0.05).Conclusions:In lead-exposed pregnant mice, the administration of hematopoietic stem cell may decrease TNF-α expression, spiral artery remodeling, and placental apoptosis index but in the group of 1/16 LD50 of lead and HSC treatment, apoptosis index has no differences with those of control group.