A comparative study to evaluate the effect of limited access dressing on diabetic ulcers

作     者：Thittamaranahalli Muguregowda Honnegowda Pramod Kumar Krishnananda Prabhu Ashwini Kumar Pragna Rao E.G.Padmanabha Udupa Shobha Kamath Antony Sylvan D’Souza Krishna Kishore Mahato 

作者机构：Department of Plastic Surgery and BurnsKasturba Medical CollegeManipal 576104KarnatakaIndia Department of Plastic SurgeryKing Abdulaziz Specialist HospitalSakaka 42421Al-JoufSaudi Arabia Department of BiochemistryKasturba Medical CollegeManipal 576104KarnatakaIndia Department of Forensic MedicineKasturba Medical CollegeManipal 576104KarnatakaIndia Department of AnatomyKasturba Medical CollegeManipal 576104KarnatakaIndia Department of BiophysicsSchool of Life SciencesManipal 576104KarnatakaIndia 

出 版 物：《Plastic and Aesthetic Research》 (整形与美容研究（英文版）)

年 卷 期：2015年第2卷第1期

页      面：266-271页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Antioxidants diabetic wounds hydroxyproline limited access dressing matrix metalloproteinases nitric oxide reactive oxygen species and oxidative stress biomarker total protein 

摘      要：Aim:Emerging evidence favors the important role of antioxidants,matrix metalloproteinases(MMPs),and nitric oxide(NO)in the healing of diabetic *** is a lack of substantial evidence regarding the effects of negative pressure on antioxidants,MMPs and NO in chronic wounds associated with ***:A total of 55 type 2 diabetic patients with leg ulcers were divided into 2 groups:a limited access dressing(LAD)group(n=27)and a conventional dressing group(n=28).Levels of hydroxyproline,total protein,MMP-2 and MMP-9,NO and antioxidants including reduced glutathione(GSH)and the oxidative biomarker malondialdehyde(MDA)were measured in the granulation tissue at days 0 and *** in levels between the LAD and conventional groups were determined by the Student’s ***:After 10 days of treatment,the LAD *** dressing group showed increase in the levels of hydroxyproline(mean±standard deviation=55.2±25.1 vs.29.2±1,P0.05),total protein(12.8±6.5 vs.8.34±3.2,P0.05),NO(1.13±0.52 vs.0.66±0.43,P0.05),GSH(7.0±2.4 vs.6.6±2.2,P0.05)and decrease in MMP-2(0.47±0.33 vs.0.62±0.30,P0.05),MMP-9(0.32±0.20 vs.0.53±0.39,P0.05)and MDA(6.8±2.3 vs.10.4±3.4,P0.05).Conclusion:When compared to conventional dressings,LAD induces biochemical changes by significantly increasing the levels of hydroxyproline,total protein,NO and antioxidants levels,and significantly reducing MMPs(MMP-2 and MMP-9)and an oxidative biomarker in diabetic *** biochemical changes are thought to favor diabetic wound healing.