Effect of repeated gonadotropin stimulation on ovarian reserves and proliferation of ovarian surface epithelium in mice
Effect of repeated gonadotropin stimulation on ovarian reserves and proliferation of ovarian surface epithelium in mice作者机构：Reproductive Medicine CenterTongji HospitalTongji Medicine CollegeHuazhong University of Science and TechnologyWuhan 430030China
出 版 物：《Frontiers of Medicine》 (医学前沿（英文版）)
年 卷 期：2009年第3卷第2期
页 面：220-226页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：gonadotropin-releasing hormone ovarian reserve embryo developmental ability ovarian surface epithelium
摘 要：This study aimed to evaluate the effect of repeated ovarian stimulation(OS)on the ovarian follicular population and morphology in female mice and its influence on the embryo’s developmental ability,and the profile of the ovarian surface epithelium(OSE).A total of 75 mice were enrolled in this experiment and randomly assigned into three groups:repeated ovarian stimulated group[n=25;receiving 5 IU pregnant mare serum gonadotrophin(PMSG)and human chorionic gonadotro-pin(hCG)at 6 day intervals for 5 cycles];single ovarian stimulated group(n=25;receiving 5 IU PMSG and hCG for 1 cycle),and control group(n=25;without additional treatment).The follicle number at various stages and the morphologies were recorded respectively in the three *** harvested oocytes or embryos,cleavage rate,good quality embryo rate,and blastocyst production rate were counted and calculated,and the proliferations of ovarian surface epithelium were evaluated *** the three groups,the single ovarian stimulation treatment significantly increased the mean number of ovarian oocytes or embryos(39.25�10.77 one-cell embryos/female);on the other hand,repeated gonadotropin stimulation obtained the lowest mean number(5.15�2.81 eggs/female,P0.01).Repeated ovarian stimulation also tended to decrease normal follicles of primary follicles(66.67%)and secondary follicles(72.86%),and got the lowest cleavage rate(67.47%),lowest good quality embryo rate(2.41%),and lowest blastocyst production rate(0).The OSE cells adjacent to the antral follicles and corpus luteum(CL)in the repeated ovarian stimulated group(81.8%)had a significantly higher proliferation rate than the other *** proliferation rate of the OSE in the single ovarian stimulated group(56.4%)was signifi-cantly higher than that in the control group(37.5%)(P0.01).In conclusion,single ovarian stimulation may produce more oocytes/***,repeated gona-dotropin stimulation may have a negative effect on the ovarian follicular quality,th
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