Beneficial effects of splenectomy on liver regeneration in a rat model of massive hepatectomy

作     者：Vikram Raut 

作者机构：Department of Breast SurgeryGraduate School of MedicineKyoto University 

出 版 物：《Hepatobiliary & Pancreatic Diseases International》 (国际肝胆胰疾病杂志（英文版）)

年 卷 期：2012年第11卷第1期

页      面：60-65页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：supported by grants from the National Natural Science Foundation of China (30772094) the State Scholarship Foundation of China (2009659015) 

主　　题：liver regeneration splenectomy hepatic oxygen delivery hepatic oxygen consumption 

摘      要：BACKGROUND: Small-for-size syndrome is a widely recognized clinical complication after living donor liver transplantation or extended hepatectomy due to inadequate liver mass. The purpose of this study was to investigate the role of splenectomy in rats after massive hepatectomy, a surrogate model of small-for-size graft. METHODS: Rats were divided into eight groups, each with 20 animals: 50% hepatectomy (50% Hx), 50% hepatectomy+ splenectomy (50% Hx+Sp), 60% Hx, 60% Hx+Sp, 70% Hx, 70% Hx+Sp, 90% Hx and 90% Hx+Sp. The following parameters were evaluated: liver function tests (ALT, AST and TBIL), liver regeneration ratio, DNA synthesis, proliferation cell nuclear antigen, hepatic oxygen delivery (HDO(2)) and hepatic oxygen consumption (HVO(2)). RESULTS: The liver regeneration ratio was enhanced in the Hx+Sp groups (P 0.05). In addition, compared with the Hx groups, the Hx+Sp groups had better liver functions (P 0.05). DNA synthesis and proliferation cell nuclear antigen were also increased in the Hx+Sp groups compared with the Hx groups (P 0.05). Furthermore, in the Hx+Sp groups, HDO(2) and HVO(2) were increased over those in the Hx groups (P 0.05), and were positively correlated with the liver regeneration ratio. CONCLUSIONS: Splenectomy significantly improved liver function, and enhanced DNA synthesis and proliferation cell nuclear antigen after massive hepatectomy in rats. This operation could be mediated through increased HDO(2), and HVO(2) which facilitate liver regeneration. (Hepatobiliary Pancreat Dis Int 2012;11:60-65)