Characterization of Nitrogen Metabolism in the Low-Nitrogen Tolerant lnt1 Mutant of Arabidopsis thaliana Under Nitrogen Stress

作     者：TENG Yi-Bo LI Ya-Juan FANG Ping LA Gui-Xiao TENG Yi-Bo1,LI Ya-Juan2,FANG Ping1,2,*2 and LA Gui-Xiao1 1Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health,College of Environmental and Resource Sciences,Zhejiang University,Hangzhou 310029(China) 2Zhejiang Provincial Key Laboratory of Subtropical Soil Science and Plant Nutrition,College of Environmental and Resource Sciences,Zhejiang University,Hangzhou 310029(China)

作者机构：Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health College of Environmental and Resource Sciences Zhejiang University Hangzhou 310029 (China) Zhejiang Provincial Key Laboratory of Subtropical Soil Science and Plant Nutrition College of Environmental and Resource Sciences Zhejiang University Hangzhou 310029 (China) 

出 版 物：《Pedosphere》 (土壤圈（英文版）)

年 卷 期：2010年第20卷第5期

页      面：623-632页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：Supported by the National Basic Research Program of China(No.2007CB109305) the National Natural Science Foundation of China(No.30370839) 

主　　题：ammonium free amino acids glutamate synthase glutamine synthetase nitrogen assimilation 

摘      要：A growth experiment on agar medium and a hydroponics experiment were carried out to study the nitrogen （N） metabolism of a low-N tolerant mutant （lntl） of Arabidopsis thaliana under different N levels as compared with the wild- type （WT） Arabidopsis. On the agar medium, no apparent growth differences were observed between the lntl and WT plants under a normal N level of 9 mmol L^-1 NO3. However, under a low N level of 0.18 mmol L^-1 NO3^-, the growth of the WT plants was greatly retarded, while the lntl plants were not affected by low-N stress and showed similar growth with those grown under a normal N level. In the hydroponics experiment, the lntl mutant had higher activities of glutamine synthetase （GS） and NADH-dependent glutamate synthase （NADH-GOGAT） in both leaves and roots under N-deficient conditions. Moreover, they accumulated less ammonium （NH4^＋） but more free amino acids in leaves compared with the WT plants. These observations suggest that better N assimilation might contribute to the low-N tolerant phenotype of the lnt1 mutant.