Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity

作     者：SUN Yuhao CHEN Xiaolin LIU Song YU Uuahua LI Rongfeng WANG Xueqin QIN Yukun LI Pengcheng 孙雨豪;陈晓琳;刘松;于华华;李荣锋;王雪芹;秦玉坤;李鹏程

作者机构：Institute of OceanologyChinese Academy of SciencesQingdao 266071China University of Chinese Academy of SciencesBeijing 100049China 

出 版 物：《Journal of Oceanology and Limnology》 (海洋湖沼学报（英文）)

年 卷 期：2018年第36卷第3期

页      面：882-891页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 10[医学] 

基　　金：Supported by the Ocean Public Welfare Scientifi c Research Project,State Oceanic Administration of the People’s Republic of China(No.201505033) the Qingdao People’s Livelihood Science and Technology Program(No.16-6-2-41-nsh) 

主　　题：anticoagulant activity Sargassum fusiforme low molecular weight degradation 

摘      要：Heparin has been used as an anticoagulant drug for many years, but it has significant side ef fects. In the search for good substitutes, low molecular weight(MW) polysaccharides from S argassum fusiforme have been examined and confi rmed to possess biological activities. Here, S. fusiforme polysaccharides(SFP) were extracted and subjected to a hydrogen peroxide(H_2O_2) oxidation method for the preparation of low-MW SFP(LSFP). The effects of temperature, pH, and H_2O_2 concentration on the degradation process were also examined. Several LSFP of 36, 9, 5.7, and 2.7 kDa were obtained under different conditions, and their anticoagulant activities studied in vitro. The results showed that SFP and LSFP prolonged activated partial thromboplastin(APTT), prothrombin(PT) and thrombin times(TT) significantly, indicating that these low MW polysaccharides possessed anticoagulant activity in the intrinsic, extrinsic, and common coagulation pathways. As these ef fects were related to the MW of the polysaccharides in APTT and TT but not in PT, the contents of the monosaccharide fucose and sulfate and the polysaccharide MW could have exerted combined ef fects. The details of this mechanism require further verifi cation.