Comparative study of responses in the brown algae Sargassum thunbergii to zinc and cadmium stress

作     者：LU Fang DIND Gang LIU Wei ZHAN Dongmei WU Haiyi GUO We 吕芳;丁刚;刘玮;詹冬梅;吴海一;郭文

作者机构：Marine Biology Institute of Shandong ProvinceQingdao 266104China Qingdao Macroalgae Engineering Technology Research CenterQingdao 266104China 

出 版 物：《Journal of Oceanology and Limnology》 (海洋湖沼学报（英文）)

年 卷 期：2018年第36卷第3期

页      面：933-941页

核心收录：

学科分类：12[管理学] 083002[工学-环境工程] 1204[管理学-公共管理] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 082803[工学-农业生物环境与能源工程] 08[工学] 0828[工学-农业工程] 120405[管理学-土地资源管理] 

基　　金：Supported by the National Natural Science Foundation of China(No.41306122) the National Special Research Fund for Non-Profit Marine Sector(Nos.201405040,201505022) the Scientific Funds for Outstanding Young Scientists of Shandong Province Award(No.BS2012HZ013) the Shandong Agricultural Application Technology Innovation of Research Project 

主　　题：Sargassum thunbergii heavy metal stress chlorophyll photosynthesis rate respiration rate rbcL gene 

摘      要：Heavy metal pollution in aquatic system is becoming a serious problem worldwide. In this study, responses of Sargassum thunbergii to dif ferent concentrations(0, 0.1, 0.5, 1.0 and 5.0 mg/L) of zinc(Zn) and cadmium(Cd) exposure separately were studied for 15 days in laboratory-controlled conditions. The results show that the specifi c growth rates increased slightly under the lower Zn concentration treatment(0.1 mg/L) at the first 5 d and then decreased gradually, which were significantly reduced with the exposure time in higher Zn concentrations and all Cd treatments compared to respective control, especially for 1.0 and 5.0 mg/L Cd. Chlorophyll a contents showed significant increase in 0.1 mg/L Zn treatment, whereas the gradually reduction were observed in the other three Zn treatments and all Cd treatments. The oxygen evolution rate and respiration rate presented distinct behavior in the Zn-treated samples, but both declined steadily with the exposure time in Cd treatments. The P/R value analyses showed similar variation patterns as chlorophyll a contents. Real-time PCR showed that lower Zn concentration(0.1 mg/L) increased mRNA expression of rbcL gene, whereas higher Zn concentrations and Cd reduced the rbcL expression. Taken together, these findings strongly indicate that Zn and Cd had different effects on S. thunbergii both at the physiological and gene transcription levels, the transcript level of photosynthesis-related gene rbcL can be used as an useful molecular marker of algal growth and environment impacts.