Arabidopsis thaliana NOP10 is required for gametophyte formation

作     者：Lin-Xiao Li Hong-Ze Liao Li-Xi Jiang Qing Tan De Ye Xue-Qin Zhang 

作者机构：State Key Laboratory of Plant Physiology and Biochemistry College of Biological Sciences China Agricultural University State Key Laboratory of Non-Food Biomass Energy and Enzyme Technology National Engineering Research Center for Non-Food Biorefinery and Guangxi Key Laboratory of Biorefinery Guangxi Academy of Sciences College of Agriculture & Biotechnology Zhejiang University 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2018年第60卷第8期

页      面：723-736页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：financially supported by the National Natural Science Foundation (31770353 and 30530060) 

主　　题：Figure Arabidopsis thaliana NOP10 is required for gametophyte formation 

摘      要：The female gametophyte is crucial for sexual reproduction of higher plants, yet little is known about the molecular mechanisms underlying its development. Here, we report that Arabidopsis thaliana NOP10 （AtNOP10） is required for female gametophyte formation. AtNOP10 was expressed predominantly in the seedling and reproductive tissues, including anthers, pollen grains, and ovules. Mutations in AtNOP10 interrupted mitosis of the functional megaspore during early development and prevented polar nuclear fusion in the embryo sacs. AtNOP10 shares a high level of amino acid sequence similarity with Saccharornycescerevisiae （yeast） NOPIo （ScNOP1o）, an important compo- nent of the H/ACA small nucleolar ribonucleoprotein particles （H/ACA snoRNPs） implicated in 18S rRNA synthe- sis and rRNA pseudouridylation. Heterologous expression of ScNOP10 complemented the mutant phenotype of Atnop10. Thus, AtNOPIo influences functional megaspore mitosis and polar nuclear fusion during gametophyte formation in Arabidopsis.