Microstreaming velocity field and shear stress created by an oscillating encapsulated microbubble near a cell membrane

作     者：王莉 屠娟 郭霞生 许迪 章东 

作者机构：Institute of Acoustics Key Laboratory of Modern Acoustics Ministry of Education Nanjing University The First Affiliated Hospital of Nanjing Medical University 

出 版 物：《Chinese Physics B》 (中国物理B（英文版）)

年 卷 期：2014年第23卷第12期

页      面：270-276页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0704[理学-天文学] 

基　　金：Projects supported by the National Basic Research Program,China(Grant No.2011CB707900) the National Natural Science Foundation of China(Grant Nos.81127901,81227004,81271589,11374155,11161120324,11074123,11174141,11274170,11104140,11474001,and 11474161) the National High Tech Research and Development Program,China(Grant No.2012AA022702) the Program for New Century Excellent Talents in University of Ministry of Education of China(Grant No.NCET-11-0236) 

主　　题：microstreaming shear stress sonoporation encapsulated microbubbles 

摘      要：Sonoporation mediated by microbubbles is being extensively studied as a promising technology to facilitate gene/drug delivery to cells. However, the theoretical study regarding the mechanisms involved in sonoporation is still in its infancy.Microstreaming generated by pulsating microbubble near the cell membrane is regarded as one of the most important mechanisms in the sonoporation process. Here, based on an encapsulated microbubble dynamic model with considering nonlinear rheological effects of both shell elasticity and viscosity, the microstreaming velocity field and shear stress generated by an oscillating microbubble near the cell membrane are theoretically simulated. Some factors that might affect the behaviors of microstreaming are thoroughly investigated, including the distance between the bubble center and cell membrane（d）, shell elasticity（χ）, and shell viscosity（κ）. The results show that（i） the presence of cell membrane can result in asymmetric microstreaming velocity field, while the constrained effect of the membrane wall decays with increasing the bubble-cell distance;（ii） the bubble resonance frequency increases with the increase in d and χ, and the decrease in κ,although it is more dominated by the variation of shell elasticity; and（iii） the maximal microstreaming shear stress on the cell membrane increases rapidly with reducing the d, χ, and κ. The results suggest that microbubbles with softer and less viscous shell materials might be preferred to achieve more efficient sonoporation outcomes, and it is better to have bubbles located in the immediate vicinity of the cell membrane.