Determination of Hesperidin and Limonin Levels as Part of the Defense Mechanism of Some Lemon Varieties [Citrus limon （L,） Burm f.] against Mal Secco Disease [Phoma tracheiphila （Petri） Kanc,et Ghik,]
Determination of Hesperidin and Limonin Levels as Part of the Defense Mechanism of Some Lemon Varieties [Citrus limon （L,） Burm f.] against Mal Secco Disease [Phoma tracheiphila （Petri） Kanc,et Ghik,]作者机构：Subtropical Fruits Research and Experimental Center Cukurova University Balcah Adana 01330 Turkey University of Cukurova Biotechnology Research and Experimental Center Balcah Adana 01330 Turkey
出 版 物：《Journal of Agricultural Science and Technology(A)》 (农业科学与技术（A）)
年 卷 期：2011年第1卷第2X期
页 面：149-155页
学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 08[工学] 09[农学] 083203[工学-农产品加工及贮藏工程]
主 题：HPLC Phoma tracheiphila Citrus limon hesperidin limonin.
摘 要：In this paper, it was aimed to identified and quantified hesperidin and limonin compounds using HPLC （High Performance Liquid Chromatography） techniques against to developing of mal secco disease caused by Phoma tracheiphila. Six citrus lemon varieties （Meyer, Kiitdiken, Enterdonato, Yediveren, Sweet lemon and Euroka） were infected by P. tracheiphila and artificial inoculation were applied in vivo conditions. Before and after inoculation, leaf, branch and stem samples were taken from each lemon varieties. The results show that the amount of hesperidin and limonin concentration was increased after the inoculations at various levels based on the lemon cultivars. Various concentrations （1, 5, 10, 25, 50, 75, 100 ppm） of hesperidin and limonin compounds were also tested under in vitro conditions to compare response of P. tracheiphila development. According to the results, hesperidin and limonin compounds play an important role against to P. tracheiphila development and Sweet Lemon variety was found to be the most resistance both observation and HPLC results.
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