The action of medicinal animal horns on Escherichia coli growth investigated by microcalorimetry and chemometric analysis

作     者：YAN Dan HAN YuMei LUO JiaoYang ZHANG Ping TANG HuiYing PENG Cheng XIAO XiaoHe 

作者机构：Institute of Chinese Medicine 302 Hospital of People's Liberation Army Beijing 100039 China Chengdu University of Traditional Chinese Medicine Chengdu 610075 China Chongqing Institute of Technology Chongqing 400050 China 

出 版 物：《Chinese Science Bulletin》 (中国科学通报)

年 卷 期：2010年第55卷第26期

页      面：2945-2950页

核心收录：

学科分类：081704[工学-应用化学] 0907[农学-林学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0829[工学-林业工程] 09[农学] 070302[理学-分析化学] 0703[理学-化学] 

基　　金：supported by the National Natural Science Foundation of China (30625042 and 30873385) the Key Technology of the National Great New Drugs Development Project of China (2009ZX09502-003 and 2009ZX09308-005) 

主　　题：生长变化规律 化学计量学 药用动物 大肠杆菌 微生物生长 相似性评估 热力学参数 生物活性评价 

摘      要：The action of Cornu Cervi Pantotrichum (CCP), Cornu Cervi (CC) and Cornu Saigae Tataricae (CST) on Escherichia coli growth were investigated using microcalorimetry to find the heat change regularity of microbial growth. The similarity of thermogenic curves and thermodynamics parameters were investigated as evaluation index, such as the growth rate constant in the first expo- nential phase (k1), maximum power in the first exponential phase (P1), maximum power in the secondary exponential phase (P2), peak time in the first exponential phase (T1), peak time in the stationary phase (T2) and the total heat production in stage 1 (Q1), and the total heat production in stage 2 (Q2). Chemometric analysis was used as a reference for the bioactivity evaluation of medicinal animal horns. The results indicated that the similarity between CST and the control was smaller than that between CCP, CC and the control. Both CCP and CC could increase the heat in the microbial growth, whereas CST decreased it. The biotic thermal activity of different medicinal animal horns was objectively, qualitatively, and quantitatively evaluated by the similarity of thermogenic curves and thermodynamics parameters analysis.