Apoptosis of endothelial cells of cerebral basilar arteries in symptomatic cerebral vasospasm rabbit models Electron microscopic observation

作     者：Guojun Shi Guodong Gao Zhenwei Zha 

作者机构：Fifth Endemic Area the 89 Hospital of Chinese PLA Weifang 261021 Shandong Province China Minimally Invasive Neurosurgical Center Tangdu Hospital Fourth Military Medical University of Chinese PLA Xi＇an 710038 ShaanxiProvince China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2007年第2卷第8期

页      面：479-482页

核心收录：

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

主　　题：encephalomyelitis models, animal autoimmunity 

摘      要：BACKGROUND： Recent researchers report that vasospasm is caused by that, on one hand, damage of endothelial cells reduces synthesis and liberation of vessel dilator; on the other hand, defluxion of endothelial cells directly exposure vascular smooth muscles in active materials of vasoconstriction in blood. OBJECTIVE： To study whether apoptosis of cerebrovascular cells occurs in symptomatic cerebral vasospasm （CVS） rabbit models by using transmission electron microscope. DESIGN： Contrast observation. SETTINGS： The Fifth Endemic Area, the 89 Hospital of Chinese PLA; Minimally Invasive Neurosurgical Center, Tangdu Hospital, the Fourth Military Medical University of Chinese PLA. MATERIALS： A total of 24 New Zealand rabbits, of either sex, weighing 2.4 - 3.0 kg, of clear grade, were selected from the Experimental Animal Center of the Fourth Military Medical University of Chinese PLA. JEM-2000EX transmission electron microscope was made in Japan. METHODS： The experiment was carried out in the Laboratory of Anatomy （National Key Laboratory）, the Fourth Military Medical University of Chinese PLA from April 2001 to April 2002. ①Preparation of symptomatic CVS models： Eighteen animals which were successfully modeled were randomly divided into experimental group （n =13） and control group （n =5）. Animals in the experimental group were poured with blood into cavitas subarachnoidealis; while, animals in the control group were poured with the same volume of saline into cavitas subarachnoidealis. At the 5^th day injection, three rabbits selected from the experimental group were anesthetized and perfused into left ventricle. And then, aorta pectoralis and caval vein were blocked by using ring clamp. Cranium was rapidly cut open to obtain cerebral basilar artery and a few of brain tissues. Both of them were fixed for 8 hours. Two rabbits selected from the control group were perfused with the same method to obtain basilar artery and brain tissues and fix. ②After fixation by using optic