Hepatic lipid metabolism changes in short-and long-term prehepatic portal hypertensive rats

作     者：Maria-Angeles Aller Elena Vara Cruz García Maria-Paz Nava Alejandra Angulo Fernando Sánchez-Patán Ana Calderón Patri Vergara Jaime Arias 

作者机构：SurgeryⅠ Department School of Medicine Complutense University of Madrid Spain Biochemical and Molecular Biology Department School of Medicine Complutense University of Madrid Spain Animal Physiology Department (Physiology Ⅱ) School of Biological Sciences Complutense University of Madrid Spain Physiology Unit. Veterinary School Autonoma University of Barcelona Bellaterra Catalua Spain 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2006年第12卷第42期

页      面：6828-6834页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by grants from Foundation MMA (SV-O4-FMM-02)  and Consejería de Sanidad  Instituto Ciencias de la Salud  de la Junta de Castilla-La Mancha (Ref. No. 04047-00) 

主　　题：Portal hypertension Free fatty acids Diacylglycerides Triglycerides Phospholipids Cholesterol 

摘      要：AIM: To verify the impairment of the hepatic lipid metabolism in prehepatic portal hypertension. METHODS: The concentrations of free fatty acids, diacylglycerol, triglycerides, and phospholipids were assayed by using D-[U-14C] glucose incorporation in the different lipid fractions and thin-layer chromatography and cholesterol was measured by spectrophotometry, in liver samples of Wistar rats with partial portal vein ligation at short- (1 mo) and long-term (1 year) (i.e. portal hypertensive rats) and the control rats. RESULTS: In the portal hypertensive rats, liver phospholipid synthesis significantly decreased (7.42 ± 0.50 vs 4.70 ± 0.44 nCi/g protein; P 0.01) and was associated with an increased synthesis of free fatty acids (2.08 ± 0.14 vs 3.36 ± 0.33 nCi/g protein; P 0.05), diacylglycerol (1.93 ± 0.2 vs 2.26 ± 0.28 nCi/g protein), triglycerides (2.40 ± 0.30 vs 4.49 ± 0.15 nCi/g protein) and cholesterol (24.28 ± 2.12 vs 57.66 ± 3.26 mg/g protein; P 0.01). CONCLUSION: Prehepatic portal hypertension in rats impairs the liver lipid metabolism. This impairment consists in an increase in lipid deposits (triglycerides,diacylglycerol and cholesterol) in the liver, accompanied by a decrease in phospholipid synthesis.