Control of MSC Differentiation by Tuning the Alkyl Chain Length of Phe- nylboroinc Acid Based Low-molecular-weight Gelators
Control of MSC Differentiation by Tuning the Alkyl Chain Length of Phe- nylboroinc Acid Based Low-molecular-weight Gelators作者机构：National Engineering Research Centre for Biomaterials Sichuan University Chengdu 610064 China College of Chemistry and Materials Engineering Wenzhou University Wenzhou 325027 China
出 版 物：《Journal of Bionic Engineering》 (仿生工程学报（英文版）)
年 卷 期：2018年第15卷第4期
页 面：682-692页
核心收录：
学科分类：081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 0822[工学-轻工技术与工程]
基 金：This work was supported by the Natural Science Foundation grants (Nos. 31600765 and 21672164) Natural Science Foundation of Zhejiang Province (No.LY 15B020001) Sichuan Province Miaozi Project (No. 2016RZ0032) and Chinese Postdoctoral Science Foundation (2016M062690)
主 题：low-molecular-weight gels phenylboronic acid alkyl chain mesenchymal stem cell osteoblast differentiation chondrocytic differentiation
摘 要：The physical environment plays a critical role in modulating stem cell differentiation into specific lineages. In this study, we designed and synthesized a series of low-molecular-weight gels （LMWGs） with different moduli based on phenylboronic acid derivatives. The moduli of the LMWGs were readily tuned by varying the alkyl chain without any chemical crosslinker. The cell responses to the gels were evaluated with mesenchymal stem cell （MSCs）, in respect of cell morphology, proliferation and differentiation. The prepared gels were non-toxic to MSCs, suggesting good biocompatibility. The hydrogel stiffness exerted a striking modulation effect on MSC fate decisions, where MSCs were inclined to differentiate into osteoblasts in stiff LMWGs and into chondrocytes in soft LMWGs. The pivotal elastic modulus of the LMWGs to drive MSC differentiation into osteoblastic lineage and chondrocytic lineage were approximately 20 kPa - 40 kPa and 1 kPa - 10 kPa, respectively. Overall, our results demonstrated that the modification ofhydrogel stiffness via tuning the alkyl chain was a simple but effective approach to regulate MSC differentiation into specific lineage, which might have important implications in the design of LMWGs for tissue engineering applications.
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