Studies on in situ PCR Detected by the BrdU Antibody Technique

作     者：Zhang Xiyuan Jiang Haibo Ma Qi Yang Jianqi Li Li ila Liu Ting (College of Life Sciences, Wuhan University, Wuhan 430072, China)Yan Ju (Genetics Lab of Tong n Medical University, Wuhan 430030) 

出 版 物：《Wuhan University Journal of Natural Sciences》 (武汉大学学报（自然科学英文版）)

年 卷 期：1996年第1卷第1期

页      面：119-124页

学科分类：07[理学] 0703[理学-化学] 

主　　题：BrdU antibody technique cell in situ PCR in situ hybridization,human Y chromosome polymerasechain reaction (PCR) 

摘      要：Using the BrdU antibody technique followed by an immuno-chemical staining(BAT),the amplification o f DNA fragments specific to human Y chromosome on cell specimen slides was efficiently detected. Whether direct BrdU incorporation into PCR products or in situ hybridization with PCR products on slides, the amplified targetDNA fragments of specimen were visualized by BAT under the microscope. The availability of BAT and differencesin the sensitivity and efficiency between BAT and dig--if-dUTP labeling in cell in situ PCR were disCussed.