NEUROBIOLOGICAL EFFECTS OF NRCF DERIVED FROM DISTAL STUMPS OF MOTOR NERVE AND SENSORY NERVE AND b-FGF ON CULTURED MOTONEURON IN VITRO

作     者：张涤生 干季良 李青峰 平萍 PING Ping, LI Qing-feng,GAN Ji-liang, ZHANG Di-sheng Department of Plastic and Reconstructive surgery, The Ninth People’s Hospital, SSMU, Shanghai 200011, China

作者机构：Department of Plastic and Reconstructive surgery The Ninth People's Hospital SSMU 

出 版 物：《Journal of Shanghai Second Medical University(Foreign Language Edition)》 (上海第二医科大学学报（英文版）)

年 卷 期：2001年第13卷第2期

页      面：91-95页

学科分类：10[医学] 

主　　题：neurotropism nerve regeneration conditions fluid nerve regeneration cultured motoneuron 

摘      要：Objective: To explore the mechanism of neurotropism in peripheral nerve regeneration by assessing the bioactivity of regeneration microcircumstance on motoneurons. Methods The motor branch Of femoral nerve to quadriceps was incised and the distal stump was sutured with one-end silicone chamber. The nerve regeneration chamber fluids from distal motor nerve stumps (motor branch of femoral nerve ) (MD-NRCF) was collected 7d post-operatively, and with the same method, nerve regeneration conditioned fluids from distal stumps nerve stumps (saphenous nerve ) (SD-NRCF) was collected. The dissociated rat’s motoneurons were co-cultured with MD-NRCF, SD-NRCF, basic fibroblast growth factor (b-FGF) and serum-free medium for 72h respectively and then were photographed under phase-contrast microscope. The longest neurites and cellbody areas of motoneurons from each group were measured by cell image processing computer system. MTT colorimetric assay was also used to measure cell activation. Results The cells of MD-NRCF group had significantly longer neurites than the other 3 groups, and their activation was also superior to those of the other groups. Conclusion These results indicate that MD-NRCF has more significant neurite-promoting and neurobiological effects on motoneuron than SD-NRCF and b-FGF.