MULTIPHOTON MICROSCOPY:A NEW APPROACH IN PHYSIOLOGICAL STUDIES AND PATHOLOGICAL DIAGNOSIS FOR OPHTHALMOLOGY

作     者：CHIU-MEI HSUEH WEN LO SUNG-JAN LIN TSUNG-JEN WANG FUNG-RUNG HU HSIN-YUAN TAN CHEN-YUAN DONG 

作者机构：Department of Physics National Taiwan University Taipei 106Taiwan Institute of Biomedical Engineering National Taiwan University Taipei 106Taiwan Department of Dermatology National Taiwan University Hospital Taipei 100Taiwan Department of Ophthalmology Taipei Medical University Hospital Taipei 100Taiwan Department of Ophthalmology National Taiwan UniversityCollege of Medicine and Hospital Taipei 100Taiwan Department of Ophthalmology Chang-Gung University Linko 333Taiwan Institute of Biomedical Engineering National Taiwan University Taipei 100Taiwan 

出 版 物：《Journal of Innovative Optical Health Sciences》 (创新光学健康科学杂志（英文）)

年 卷 期：2009年第2卷第1期

页      面：45-60页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0808[工学-电气工程] 070207[理学-光学] 0809[工学-电子科学与技术（可授工学、理学学位）] 07[理学] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0836[工学-生物工程] 0803[工学-光学工程] 0702[理学-物理学] 

基　　金：support of the National Research Program of Genomic Medicine(NRPGM)of the National Science Council(NSC)in Taiwan 

主　　题：Multiphoton microscopy fluorescence second harmonic generation cornea sclera 

摘      要：Multiphoton microscopy(MPM),with the advantages of improved penetration depth,decreased photo-damage,and optical sectioning capability,has become an indispensable tool for biomedical *** combination of multiphoton fluorescence(MF)and second-harmonic generation(SHG)microscopy is particularly effective in imaging tissue structures of the ocular *** work is intended to be a review of advances that MPM has made in ophthalmic *** MPM not only can be used for the label-free imaging of ocular structures,it can also be applied for investigating the morphological alterations in corneal pathologies,such as keratoconus,infected keratitis,and corneal ***,the corneal wound healing process after refractive surgical procedures such as conductive keratoplasty(CK)can also be studied with ***,qualitative and quantitative SHG microscopy is effective for characterizing corneal thermal *** additional development,multiphoton imaging has the potential to be developed into an effective imaging technique for in vivo studies and clinical diagnosis in ophthalmology.