Surfactant-coated Candida rugosa Lipase as Catalyst for Hydrolysis of Olive Oil in Solvent-Free Two-Phase System
Surfactant-coated Candida rugosa Lipase as Catalyst for Hydrolysis of Olive Oil in Solvent-Free Two-Phase System作者机构:Chemical Engineering Research Center School of Chemical Engineering and Technology Tianjin University Tianjin 300072China Department of Chemistry & Chemical Engineering Faculty of Engineering Kanazawa University 2-40-20 Kodatsuno Kanazawa 920-8667 Japan Chemical Engineering Research Center School of Chemical Engineering and Technology Tianjin University Tianjin 300072 China
出 版 物:《Chinese Journal of Chemical Engineering》 (中国化学工程学报(英文版))
年 卷 期:2003年第11卷第5期
页 面:601-603页
核心收录:
学科分类:081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学]
基 金:National Natural Science Foundation of China(No.29876031)
主 题:Candida rugosa hydrolysis lipase olive oil solvent-free system surfactant
摘 要:The surfactant-coated Candida rugosa lipase was used as catalyst for hydrolysis of olive oil in two-phase system consisting of olive oil and phosphate buffer without organic solvent. For both the coated and native lipases,the optimal buffer/oil volume ratio of 1.0, aqueous pH 6.8 and reaction temperature 30℃ were determined. The maximum activity of the coated lipase was ca 1.3 times than that of the native lipase. The half-life of the coated lipase in olive oil and the native lipase in phosphate buffer was ca 9 h and 12 h, and the final residual activity was 27% and 20% of their initial values, respectively. The final substrate conversion by the coated lipase was ca 20% higher than that of the native lipase.