Evaluation of phenolic composition, antioxidant, anti-inflammatory and anticancer activities of Polygonatum verticillatum(L.)

作     者：SANDeep Kumar Singh Arjun Patra 

作者机构：Institute of Pharmacy Guru Ghasidas University 

出 版 物：《Journal of Integrative Medicine》 (结合医学学报（英文版）)

年 卷 期：2018年第16卷第4期

页      面：273-282页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：The financial support of University Grants Commission,New Delhi,India as Rajiv Gandhi National Fellowship[F1-17.1/2013-14/RGNF-2013-14-SC-UTT-36274/(SA-III/Website)]to SKS UGC-BSR Research Start-Up-Grant to AP[N.F.20-14/2012(BSR)] 

主　　题：Polygonatum verticillatum Gas chromatography-mass spectrometry Phenolic profile MCF-7 cells 

摘      要：Objective： Polygonaturn verticillatum （L） All. （Ruscaceae）, one of the Ashtawarga plants, is widely used for treatment of various ailments. The present study was undertaken to determine the phenolic composition, antioxidant, anti-inflammatory and anticancer activities of several extracts （petroleum ether, dichloro- methane, chloroform, ethanol, and aqueous） from the rhizomes of the plant. Methods： Coarsely powdered dry rhizome was successively extracted with different solvents of increas- ing polarity （petroleum ether, dichloromethane, chloroform, ethanol and water）. The phenolic composi- tions, in terms of total phenolic content （TPC）, total flavonoid content （TFC） and total condensed tannin content （JTC）, were evaluated with the Folin-Ciocalteu assay, aluminum chloride colorimetric assay and vanillin spectrophotometric assay, respectively. Total antioxidant capacity, 1,1-diphenyl-2- picrylhydrazyl and 2,2＇-azino-his（3-ethylbenzothiazoline-6-sulfonic acid） assays were used to assess the antioxidant potential of each extract. A protein denaturation model and 3-（4,5-dimethylthiazol-2- yl）-2,5-diphenyltetrazolium bromide assay were used to evaluate in vitro anti-inflammatory and anti- cancer activities, respectively. Gas chromatography-mass spectrometry （GC/MS） analysis was carried out to demonstrate various phytoconstituents in each extract. Correlation studies were also performed between phenolic composition （TPC, TFC and TFC） and different biological activities. Results： Ethanol extract showed maximum TPC （0.126 mg/g, gallic acid equivalent in dry sample）, TFC （0.094 mg/g, rutin equivalent in dry sample） and TTC （29.32 mg/g, catechin equivalent in dry sample）, as well as antioxidant and anti-inflammatory properties. Chloroform extract exhibited the strongest cyto- toxicity against the human breast cancer cell line, MCF-7. GC/MS analysis revealed the presence of 90 different phytoconstituents among the extracts. Antioxidant and anti-inflammatory activities had a pos