Functional non-homologous end joining patterns triggered by CRISPR/Cas9 in human cells

作     者：Fayu Yang Xianglian Ge Xiubin He Xiexie Liu Chenchen Zhou Huihui Sun Junsong Zhang Junzhao Zhao Zongming Song Jia Qu Changbao Liu Feng Gu 

作者机构：School of Ophthalmology and Optometry Eye Hospital Wenzhou Medical University State Key Laboratory and Key Laboratory of Vision Science Ministry of Health and Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry Wenzhou 325027 China The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University Wenzhou 325000 China Henan Eye Institute Henan Eye Hospital Henan Provincial People's Hospital and People's Hospital of Zhengzhou University Zhengzhou 450003 China 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2018年第45卷第6期

页      面：329-332页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0836[工学-生物工程] 

基　　金：supported by the grants from the Natural Science Foundation of China (No.81201181 to F.G 81473295 and 81670882 to Z.M.S and 81700885 to X.L.G.) Zhejiang Provincial & Ministry of Health research fund for medical sciences (WKJ2013-2-023 to F.G WKJ-ZJ-1828 to J.Z.Z.and 2016KYA145 to X.L.G.) Science Technology Project of Zhejiang Province (2014C33260 to Z.M.S.and 2017C37176 to F.G.) Eye Hospital at Wenzhou Medical University (YNZD201602 to F.G.) Wenzhou City (Y20160008 to J.Z.Z.) Research Fund for Lin He's Academician Workstation of New Medicine and Clinical Translation (17331209 to C.B.L.) 

主　　题：GFP Functional non-homologous end joining patterns triggered by CRISPR/Cas9 in human cells PCR RNA HEK 

摘      要：CRISPR/Cas9-mediated genome engineering technologies are now widely applied in various organisms,including mouse and human cells（Cong et al.,2013;Mali et al.,2013;Yang et al.,2013;Hsu et al.,2014）.The most widely used customized CRISPR/Cas9（Sp Cas9）is derived from Streptococcus pyogenes（Cong et al.,2013）.