Caudal dorsal artery generates hematopoietic stem and progenitor cells via the endothelial-to-hematopoietic transition in zebrafish

作     者：YANDong Zhan Youkui Huang Jingying Chen Zigang Cao Jianbo He Jingjing Zhang Honghui Huang Hua Ruan Lingfei Luo Li Li 

作者机构：The State Key Laboratory Breeding Base of Bioresources and Eco-environments Key Laboratory of Freshwater Fish Reproduction and Development Ministry of Education Laboratory of Molecular Developmental Biology School of Life Sciences Southwest University Chongqing 400715 China Affiliated Hospital of Guangdong Medical University Zhanjiang 524001 China 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2018年第45卷第6期

页      面：315-324页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071008[理学-发育生物学] 

基　　金：supported by the National Natural Science Foundation of China (Nos.31571500,31771623 and 31771628) the National Key Basic Research Program of China (2015CB942802) the Fundamental Research Funds for the Central Universities (XDJK2017A015) the Guangdong Natural Science Fund for Distinguished Young Scholars (2017A030306024) 

主　　题：Caudal dorsal artery Endothelial-to-hematopoietic transition Circulation Zebrafish 

摘      要：Zebrafish hematopoietic stem and progenitor cells（HSPCs） originate from the hemogenic endothelium of the ventral wall of the dorsal aorta（DA） through the endothelial-to-hematopoietic transition（EHT） from approximately 30 to 60 hours post fertilization（hpf）. However, whether other artery sites can generate HSPCs de novo remains unclear. In this study, using live imaging and lineage tracing, we found that the caudal dorsal artery（CDA） in the caudal hematopoietic tissue directly gave rise to HSPCs through *** process initiated from approximately 60 hpf and terminated at approximately 156 hpf. Compared with that in the DA, fewer EHT events were observed in the CDA. The EHT events in the DA and CDA were similarly regulated by Runx1 but differentially influenced by blood flow（i.e., the EHT frequency in CDA was affected to a lesser extent when circulation was compromised in the tnnt2a～（-/-）mutant）. Therefore,the whole artery, including both DA and CDA, was endowed with the ability to produce HSPCs during a much longer time period. Coincidently, the lineage tracing results indicated that adult hematopoietic cells originated from the embryonic endothelium, and those produced later preferentially colonized the adult thymus. Collectively, our study revealed that the CDA serves as an additional source of hematopoiesis, and it shows similar but not identical properties with the DA.