Effects of Panax notoginseng saponins on hydrogen peroxide-induced apoptosis in cultured rabbit bone marrow stromal cells

作     者：Hui Qiang1,2,Guang-Sheng Wang3,Chen Zhang1,Zhi-Bin Shi1,Li-Hong Fan1,Kun-Zheng Wang1 1.Department of Orthopedics,the Second Affiliated Hospital,Medical School of Xi’an Jiaotong University,Xi’an 710004 2.Department of Orthopedics,Shaanxi Province People’s Hospital,Xi’an 710068 3.Department of Orthopedics,Huashan Hospital,Baoji 721000,China. 

作者机构：Department of Orthopedics Second Affiliated Hospital Medical School of Xi'an Jiaotong University Xi'an 710004 China Department of Orthopedics Shaanxi Province People's Hospital Xi'an 710068 China Department of Orthopedics Huashan Hospital Baoji 721000 China 

出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)

年 卷 期：2010年第22卷第1期

页      面：25-29页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(No.30600624) 

主　　题：Panax notoginseng saponins reactive oxygen species bone marrow stromal cell apoptosis Bax 

摘      要：Objective To investigate the effects of Panax notoginseng saponins(PNS)on hydrogen peroxide(H2O2)-induced apoptosis in cultured rabbit bone marrow stromal cells(BMSCs).Methods BMSCs from 3-month-old New Zealand rabbits were isolated and cultured by the density gradient centrifugation combined with adherent *** cultured BMSCs were divided into three groups:normal control,H2O2 treatment(100μmol/L),and PNS pretreatment(0.1g/L).Intracellular reactive oxygen species(ROS)levels as the index of oxidative stress were measured by using 2’7’-dichlorodihydrofluorescein *** cytometry was used to observe the apoptosis of BMSCs by staining with annexinV-FITC/*** protein expression of Bax in BMSCs was analyzed by Western *** of caspase-3 enzyme was measured by *** Pretreatment with PNS significantly decreased intracellular ROS level induced by H2O2(P0.01).PNS markedly attenuated H2O2-induced apoptosis rate from 38.68% to 19.24%(P0.01).PNS reversed H2O2-induced augmentation of Bax ***,PNS markedly reduced the altered in activity of caspase-3 enzyme induced by H2O2(P0.01).Conclusion PNS has a protective effect on hydrogen peroxide-induced apoptosis in cultured rabbit BMSCs by scavenging ROS and decreasing Bax expression and caspase-3 activity.