Enzyme activities and gene expression of starch metabolism provide insights into grape berry development
作者机构：College of HorticultureNanjing Agricultural UniversityNo 1 weigangNanjing210095China Institute of HorticultureJiangsu Academy of Agricultural SciencesNo 50 Zhongling roadNanjing210014China
出 版 物：《Horticulture Research》 (园艺研究（英文）)
年 卷 期：2017年第4卷第1期
页 面：277-292页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：This research was financed by the Natural Science Foundation of China(NSFC)(No.31672131) Science and Technology Support Program of Jiangsu Province(CX(12)2013) Fund Project of Agricultural Science and Technology in Jiangsu Province(BE2013431)
主 题：metabolism starch elevated
摘 要：Grapes are categorized as a non-climacteric type of fruit which its ripening is not associated to important rises in respiration and ethylene *** starch metabolism shares a certain role in the carbohydrate metabolic pathways during grape berry development,and is regarded as an important transient pool in the pathway of sugar ***,the comprehensive role of starch and its contribution to the quality and flavor of grape berry have not been explored *** this study,the expression levels of genes enzyme activities and carbohydrate concentrations related to starch metabolism,were analyzed to understand the molecular mechanism of starch accumulation during grape berry *** results indicated that starch granules in grape berry were located at the chloroplast in the sub-epidermal tissues,acting as the temporary reserves of photosynthetic products to meet the needs for berry development,and relatively high starch contents could be detected at véraison ***,both ADP-glucose pyrophosphorylase(EC 2.***.7.27)and sucrose phosphate synthase(EC 2.***.1.14)involved in starch synthesis displayed elevated gene expression and enzymes activities in the sub-epidermal tissue,whileα-andβ-amylases involved in its degradation were highly transcribed and active in the central flesh,explaining the absence of starch in this last *** in the gene expression and activities of ADP-glucose pyrophosphorylase,β-amylase and sucrose phosphate synthase revealed that they were regulated by the circadian rhythms in the fruitlets compared with those in the *** the morphological,enzymological and transcriptional data in this study provide advanced understandings on the function of starch during berry development and ripening that are so important for berry *** study will further facilitate our understanding of the sugar metabolism in grape berry as well as in other plant species.
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