Mutagenesis and selection of high efficiency hydrogen-producing mutants by ultraviolet radiation

作     者：任南琪 郑国香 李永峰 林海龙 李建政 

作者机构：School of Municipal and Environmental EngineeringHarbin Institute of Technology 

出 版 物：《Journal of Harbin Institute of Technology(New Series)》 (哈尔滨工业大学学报（英文版）)

年 卷 期：2006年第13卷第6期

页      面：635-639页

核心收录：

学科分类：07[理学] 09[农学] 0903[农学-农业资源与环境] 0713[理学-生态学] 

基　　金：Sponsored by"973"Fundamental Science Program of China(Grant No. G2000026402) and National Natural Science Fund of China (Grant No. 30470054) 

主　　题：biohydrogen UV-mutagenesis mutants 

摘      要：Hydrogen is an ideal, clean and sustainable energy source for the future because of its high conversion and nonpolluting nature. Biohydrogen production by dark-fermentation appears to have a great potential to be developed for practical application. However, one limiting factor affecting the development of hydrogen-production industrialization is that the hydrogen-producing capacity of bacteria is lower, so how to increase bacteria＇ s hydrogen-producing ability will be an urgent issue. In this experiment, 2 mutants, namely UV3 and UV7, were obtained by ultra-violet radiation. They grew and produced hydrogen efficiently on iron-containing medium. The hydrogen evolution of UV3 and UV7 were 2 356. 68 ml/L and 2 219. 62 ml/L at a glucose concentration of 10 g/L, respectively. With wild parent strain Ethanoligenens sp. ZGX4, the hydrogen evolution was 1 806. 02 ml/L under the same conditions. Mutants＇ hydrogen-producing capacities were about 29. 71% and 22.22% higher than that of wild parent strain ZGX4. The maximum H2 production rate by mutants UV3 and UV7 were estimated to be 32.57 mmol H2/g cell h and 31.19 mmol H2/g cell h, respectively, which were 38. 18% and 34. 78% higher than the control （23.57 mmol H：/g cell h）. The abundant products of UV3 and UV7 were ethanol and acetic, which accounted for 95% - 98% of total soluble microbial products. In each case, mutant strains UV3 and UV7 evolved hydrogen at a higher rate than the wild type, showing a possible potential for commercial hydrogen production. Another mutant named UV20＇ was also gained whose main end metabolites were butyric acid and acetic acid. This would provide researched material for a discussion of metabolic pathways of hydrogen-producing bacteria.