MONOCLONAL ANTIBODY 55 (CD10) AND COMPLEMENT USED FOR PURGING AUTOLOGOUS BONE MARROW IN COMMON ACUTE LYMPHOBLASTIC LEUKEMIA
作者机构：Institute of Basic Medical Sciences Academy of Military Medical Sciences Beijing.
出 版 物：《中华医学杂志(英文版)》 (Chinese Medical Journal)
年 卷 期：1990年第103卷第5期
页 面：385-390页
核心收录：
学科分类：1002[医学-临床医学]
摘 要：A CD10 monoclonal antibody 55 (McAb55) was intended for purging residual common acute lymphoblastic leukemia antigen (CALLA) positive leukemic cells from autotransplants of common acute lymphoblastic leukemia (C-ALL) patients. It was found that after two rounds of McAb55 and complement treatment, 4-5 logs of CALLA+ cells were removed from bone marrow detected by clonogenic assay. The standardization of separation, purgation and preservation of bone marrow for C-ALL patients’ autotransplants was then set as follows: Following the carboxymethyl starch sedimentation and Ficoll-Hypaque gradient separation, the isolated mononuclear cells (MNCs) were treated with McAb55 and complement twice and kept in room temperature for 48-72 hours prior to infusion. This procedure resulted in the remoral of more than 99% of CALLA+ cells, recovery of 10-30% MNCs, and leaving the hematopoiesis stem cells intact. After the intensive cytoreductive therapy, 4 patients with C-ALL received the purged autotransplants giving timely recovery of the hemmatopoietic function. The patients were all remaining in remission status for more than 40-250 days so far.
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