Recent advances in enzyme-mediated peptide ligation

作     者：Silin Xu Zhenguang Zhao Junfeng Zhao 

作者机构：Key Laboratory of Chemical Biology of Jiangxi ProvinceCollege of Chemistry and Chemical EngineeringJiangxi Normal University The Institute of ChemistryThe Hebrew University of Jerusalem State Key Laboratory of Elemento-Organic ChemistryNankai University 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2018年第29卷第7期

页      面：1009-1016页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：The National Natural Science Foundation of China (Nos. 21462023, 21778025) the Education Department of Jiangxi Province (No.150297) 

主　　题：Ligase Peptide synthesis Protein engineering Enzyme-mediated peptide ligation Site-specific protein modification 

摘      要：Artificial synthesis and site-specific modification of peptides and proteins has evolved into an indispensable tool for protein engineers and chemical biologists. Chemical and enzymatic approaches to peptide ligation are important alternatives of recombinant DNA technology for protein synthesis and modification. Although as old as that of chemical procedures, enzyme-mediated peptide ligation is far less developed than that of chemical counterpart due to the difficult availability of peptide ***, this situation has been changed slowly with the fast development of biological techniques. In the past decades, several natural peptide ligases have been discovered. Protein engineering to improve the ligation efficiencies of the natural peptide ligase and to reverse the functionality of protease provide more powerful peptide ligases. In this review, the advances of enzyme-mediated peptide ligation and their application in protein synthesis and modification will be discussed.