Assessment of mouse BMMNC DNA damage with a two-tailed comet assay after X-ray and carbon ion total body irradiation

作     者：Fang Liu Zhuan-Zi Wang Wen-Jian Li Wei Wei Bing-Rong Dang 

作者机构：Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000 China University of Chinese Academy of Sciences Beijing 100049China 

出 版 物：《Nuclear Science and Techniques》 (核技术（英文）)

年 卷 期：2018年第29卷第6期

页      面：1-8页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 100207[医学-影像医学与核医学] 1006[医学-中西医结合] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 08[工学] 1010[医学-医学技术（可授医学、理学学位）] 100106[医学-放射医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(No.11575259) 

主　　题：DNA 碳离子 损坏 彗星 尾巴 身体 金 鼠标 

摘      要：The two-tailed comet assay(2T-comet assay) is a method for simultaneously evaluating DNA single-strand breaks(SSBs) and double-strand breaks(DSBs). In the present study, the endonuclease DNase I and hydrogen peroxide were used to induce DSBs and SSBs in bone marrow mononuclear cells(BMMNCs) from mice, and the damaged DNAs were assessed with a 2T-comet assay. The results demonstrated that this method can detect and discriminate between BMMNC DNA SSBs and DSBs simultaneously. Using this method, we studied DNA damage in BMMNCs from female BALB/c mice after total body irradiation with X-rays and carbon ions. The results indicated that these two types of radiation induced serious DNA damage in BMMNCs in a dose-dependent *** DNA damage induced by carbon ions was more severe than that induced by X-rays at the same dose, and a high dose of carbon ion radiation was more likely to cause death in mice. The DSBs and SSBs induced by X-rays were the highest on the 3rd day post-IR. For carbon ion radiation,DSBs were the most serious on the 3rd day, while SSBs were more obvious on the 3rd day and 13th day *** ratio of DSBs/SSBs was clearly related to the different types of radiation.