Microsecond scale vibrational spectroscopic imaging by multiplex stimulated Raman scattering microscopy
作者机构：Weldon School of Biomedical EngineeringPurdue UniversityWest LafayetteIN 47907USA Department of Biological SciencesPurdue UniversityWest LafayetteIN 47907USA Department of ChemistryPurdue UniversityWest LafayetteIN 47907USA
出 版 物：《Light(Science & Applications)》 (光（科学与应用)（英文版）)
年 卷 期：2015年第4卷第1期
页 面：454-462页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：This work was supported by NIH grants GM104681 and CA182608
主 题：optical microscopy Raman scattering vibrational spectroscopy
摘 要：Real-time vibrational spectroscopic imaging is desired for monitoring cellular states and cellular processes in a label-free *** spectroscopic imaging of highly dynamic systems is inhibited by relatively slow spectral acquisition on millisecond to second ***,we report microsecond scale vibrational spectroscopic imaging by lock-in free parallel detection of spectrally dispersed stimulated Raman scattering *** a homebuilt tuned amplifier array,our method enables Raman spectral acquisition,within the window defined by the broadband pulse,at the speed of 32 μs and with close to shot-noise limited detection *** with multivariate curve resolution analysis,our platform allows compositional mapping of lipid droplets in single live cells,observation of intracellular retinoid metabolism,discrimination of fat droplets from protein-rich organelles in Caenorhabditis elegans,spectral detection of fast flowing tumor cells and monitoring drug diffusion through skin tissue in *** reported technique opens new opportunities for compositional analysis of cellular compartment in a microscope setting and high-throughput spectral profiling of single cells in a flow cytometer setting.
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