A transcription assay for EWS oncoproteins in Xenopus oocytes
A transcription assay for EWS oncoproteins in Xenopus oocytes作者机构:Department of BiologyThe Hong Kong University of Science and TechnologyClear Water BayKowloon Hong KongChina
出 版 物:《Protein & Cell》 (蛋白质与细胞(英文版))
年 卷 期:2010年第1卷第10期
页 面:927-934页
核心收录:
学科分类:0710[理学-生物学] 0831[工学-生物医学工程(可授工学、理学、医学学位)] 1007[医学-药学(可授医学、理学学位)] 0301[法学-法学] 03[法学] 1002[医学-临床医学] 0703[理学-化学] 0836[工学-生物工程]
基 金:supported by The Association for International Cancer Research(AICR)(grant 03-131 to K.A.W.L.)
主 题:EWS/ATF1 Ewing's sarcoma microinjection Xenopus oocytes transcription EWS-activation domain
摘 要:Aberrant chromosomal fusion of the Ewing s sarcoma oncogene(EWS)to several different cellular partners produces the Ewing s family of oncoproteins(EWSfusion-proteins,EFPs)and associated tumors(EFTs).EFPs are potent transcriptional activators,dependent on the N-terminal region of EWS(the EWS-activationdomain,EAD)and this function is thought to be central to EFT oncogenesis and *** EFPs are promising therapeutic targets,but detailed molecular studies will be pivotal for exploring this *** studies have so far largely been restricted to intact mammalian cells while recent evidence has indicated that a mammalian cell-free transcription system may not support bona fide EAD ***,the lack of manipulatable assays for the EAD presents a significant barrier to *** Xenopus laevis oocytes we describe a plasmid-based micro-injection assay that supports efficient,bona fide EAD transcriptional activity and hence provides a new vehicle for molecular dissection of the EAD.