A transcription assay for EWS oncoproteins in Xenopus oocytes

作     者：King Pan Ng Felix Cheung Kevin A.W.Lee 

作者机构：Department of BiologyThe Hong Kong University of Science and TechnologyClear Water BayKowloon Hong KongChina 

出 版 物：《Protein & Cell》 (蛋白质与细胞（英文版）)

年 卷 期：2010年第1卷第10期

页      面：927-934页

核心收录：

学科分类：0301[法学-法学] 03[法学] 

基　　金：supported by The Association for International Cancer Research(AICR)(grant 03-131 to K.A.W.L.) 

主　　题：EWS/ATF1 Ewing's sarcoma microinjection Xenopus oocytes transcription EWS-activation domain 

摘      要：Aberrant chromosomal fusion of the Ewing s sarcoma oncogene(EWS)to several different cellular partners produces the Ewing s family of oncoproteins(EWSfusion-proteins,EFPs)and associated tumors(EFTs).EFPs are potent transcriptional activators,dependent on the N-terminal region of EWS(the EWS-activationdomain,EAD)and this function is thought to be central to EFT oncogenesis and *** EFPs are promising therapeutic targets,but detailed molecular studies will be pivotal for exploring this *** studies have so far largely been restricted to intact mammalian cells while recent evidence has indicated that a mammalian cell-free transcription system may not support bona fide EAD ***,the lack of manipulatable assays for the EAD presents a significant barrier to *** Xenopus laevis oocytes we describe a plasmid-based micro-injection assay that supports efficient,bona fide EAD transcriptional activity and hence provides a new vehicle for molecular dissection of the EAD.