Placental growth factor expression is reversed by anti-vascular endothelial growth factor therapy under hypoxic conditions
Placental growth factor expression is reversed by anti-vascular endothelial growth factor therapy under hypoxic conditions作者机构：Department of Ophthalmologythe Second Affi liated Hospital of Medical College of Xi'an Jiaotong UniversityXi'an 710004Shaanxi ProvinceChina Key Laboratory of Vision Loss and RestorationMinistry of EducationDepartment of OphthalmologyPeople's HospitalPeking UniversityBeijingChina
出 版 物：《World Journal of Pediatrics》 (世界儿科杂志（英文版）)
年 卷 期：2014年第10卷第3期
页 面：262-270页
核心收录：
学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：supported by the National Basic Research Program of China(973 Program 2011CB510200)
主 题：human umbilical vein endothelial cells oxygen-induced retinopathy placental growth factor vascular endothelial growth factor
摘 要：Background:Clinical trials have revealed that the antivascular endothelial growth factor(VEGF)therapies are effective in retinopathy of prematurity(ROP).But the low level of VEGF was necessary as a survival signal in healthy conditions,and endogenous placental growth factor(PIGF)is redundant for *** purpose of this study was to elucidate the PIGF expression under hypoxia as well as the infl uence of anti-VEGF therapy on ***:CoCl2-induced hypoxic human umbilical vein endothelial cells(HUVECs)were used for an in vitro study,and oxygen-induced retinopathy(OIR)mice models were used for an in vivo *** expression patterns of PIGF under hypoxic conditions and the infl uence of anti-VEGF therapy on PIGF were evaluated by quantitative reverse transcription-polymerase chain reaction(RTPCR).The retinal avascular areas and neovascularization(NV)areas of anti-VEGF,anti-PIGF and combination treatments were *** PIGF concentration was evaluated by ELISA after *** vasoactive effects of exogenous PIGF on HUVECs were investigated by proliferation and migration ***:PIGF mRNA expression was reduced by hypoxia in OIR mice,in HUVECs under hypoxia and anti-VEGF ***,PIGF expression was reversed by anti-VEGF therapy in the OIR model and in HUVECs under *** PIGF significantly inhibited HUVECs proliferation and migration under normal conditions,but it stimulated cell proliferation and migration under ***-PIGF treatment was effective for neovascular tufts in OIR mice(P0.05).Conclusion:The finding that PIGF expression is iatrogenically up-regulated by anti-VEGF therapy provides a consideration to combine it with anti-PIGF therapy.
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