Effect of acupuncture in intervening heroin-induced brain damage via regulating ubiquitin-proteasome pathway

作     者：Zhang Li-da Cao Jiang-peng Cai Xing-hui WU Sheng-bing Hou Xiao-rong Gao Yong-long Zhang Rong-jun Song Xiao-ge 张利达;曹江鹏;蔡兴慧;吴生兵;侯晓蓉;高永龙;张荣军;宋小鸽

作者机构：School of GraduateAnhui University of Chinese MedicineHefei 230038China School of AcupunctureMoxibustion and TuinaAnhui University of Chinese MedicineHefei 230038China Key Laboratory of Xin＇ an Medicine Constructed by Anhui Province and Chinese Ministry of EducationAnhui University of Chinese MedicineHefei 230038China School of Integrated Traditional Chinese and Western MedicineAnhui University of Chinese MedicineHefei 230038China Institute of Acupuncture and MeridianAnhui University of Chinese MedicineHefei 230038China 

出 版 物：《Journal of Acupuncture and Tuina Science》 (针灸推拿医学（英文版）)

年 卷 期：2018年第16卷第2期

页      面：80-88页

核心收录：

学科分类：1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：国家自然科学基金项目,No.81173325,No.81503658 安徽省大学生创新训练计划项目,No.2015043 安徽高校科研平台建设项目,No.2015TD033 

主　　题：Acupuncture Therapy Ubiqutins Proteasome Substance Withdrawal Syndrome Heroin Dependence BrainInjuries Rats 

摘      要：Objective：To observe the effect of acupuncture in regulating ubiquitin-proteasome pathway （UPP）,and discuss the action of acupuncture in intervening heroin-induced brain ***：Thirty male Sprague-Dawley （SD） rats were divided into a control group,a model group and an acupuncture group by using the random number *** in the model and acupuncture groups received intramuscular heroin injection for successive 8 d at a progressively increased ***,the injection was suspended for 5 d for *** heroin relapse rat model was established by repeating the drug addiction and withdrawal process for 3 *** control group followed the step of the model establishment,but was given intramuscular injection of normal saline at the stage of addiction and no intervention at the stage of withdrawal;the model group was given intramuscular heroin injection at a progressively increased dose at the addiction stage and no intervention at the withdrawal stage;the acupuncture group was dealt in the same way as the model group at the addiction stage,but received acupuncture at Baihui （GV 20） and Dazhui （GV 14） at the withdrawal stage,with the needles retained for 30 min each time,1 session a day,for successive 5 *** the 39th day,brain tissues were extracted from the hippocampus and ventral tegmental area （VTA） of the three groups of *** apoptosis of brain nerve cells was detected by using terminal deoxynucleotidyl transferase-mediated nick and labeling （TUNEL）.The mRNA and protein expressions of ubiquitin （Ub）,ubiquitin protein ligase （E3） and 26S were examined by immunohistochemistry and quantitative real-time polymerase chain reaction （RT-qPCR）.Results：Compared with the model group,rat＇s hippocampus and VTA in the acupuncture group showed significantly fewer cells positively stained by TUNEL staining （P〈0.01）,and its mRNA and protein expressions of Ub,E3,26S were significantly lower （P〈0.01）.Conclusion：Reducing nerve cell apoptosis and regulating th