Extensions of PDZ domains as important structural and functional elements

作     者：Conan K.Wang Lifeng Pan Jia Chen Mingjie Zhang 

作者机构：Department of BiochemistryMolecular Neuroscience CenterHong Kong University of Science and TechnologyClear Water BayKowloonHong Kong Eskitis Institute for Cell and Molecular TherapiesGriffith UniversityBrisbaneQld 4111Australia 

出 版 物：《Protein & Cell》 (蛋白质与细胞（英文版）)

年 卷 期：2010年第1卷第8期

页      面：737-751页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 07[理学] 0703[理学-化学] 0836[工学-生物工程] 0701[理学-数学] 070101[理学-基础数学] 

基　　金：supported by grants from the Research Grants Council of Hong Kong to M.Z.(HKUST663407,663808,664009,CA07/08.SC01,SEG_HKUST06 and AoE/B-15/01-II) supported by a fellowship from the NHMRC of Australia(536578) 

主　　题：PDZ domain PDZ extensions protein structure 

摘      要：‘Divide and conquer’has been the guiding strategy for the study of protein structure and *** are divided into domains with each domain having a canonical structural definition depending on its *** this review,we push forward with the interesting observation that many domains have regions outside of their canonical definition that affect their structure and function;we call these regions‘extensions’.We focus on the highly abundant PDZ(PSD-95,DLG1 and ZO-1)*** bioinformatics,we find that many PDZ domains have potential extensions and we developed an openly-accessible website to display our results(http://***/pdzex/).We propose,using well-studied PDZ domains as illustrative examples,that the roles of PDZ extensions can be classified into at least four categories:1)protein dynamics-based modulation of target binding affinity,2)provision of binding sites for macro-molecular assembly,3)structural integration of multi-domain modules,and 4)expansion of the target ligand-binding *** review highlights the potential structural and functional importance of domain extensions,highlighting the significance of looking beyond the canonical boundaries of protein domains in general.