Control of Cell Wall Extensibility during Pollen Tube Growth

作     者：Peter K. Hepler Caleb M. Rounds Lawrence J. Winship 

作者机构：Biology Department University of Massachusetts Amherst MA 01003 USA School of Natural Science Hampshire College Amherst MA 01002 USA 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2013年第6卷第4期

页      面：998-1017页

核心收录：

学科分类：0710[理学-生物学] 0810[工学-信息与通信工程] 07[理学] 08[工学] 071009[理学-细胞生物学] 0901[农学-作物学] 0902[农学-园艺学] 081002[工学-信号与信息处理] 

基　　金：美国国家科学基金 

主　　题：cell expansion cell walls cytoskeleton dynamics polarity pollen development. 

摘      要：In this review, we address the question of how the tip-growing pollen tube achieves its rapid rate of elongation while maintaining an intact cell wall. Although turgor is essential for growth to occur, the local expansion rate is controlled by local changes in the viscosity of the apical wall. We focus on several different structures and underly- ing processes that are thought to be major participants including exocytosis, the organization and activity of the actin cytoskeleton, calcium and proton physiology, and cellular energetics. We think that the actin cytoskeleton, in particular the apical cortical actin fringe, directs the flow of vesicles to the apical domain, where they fuse with the plasma mem- brane and contribute their contents to the expanding cell wall. While pH gradients, as generated by a proton-ATPase located on the plasma membrane along the side of the clear zone, may regulate rapid actin turnover and new polymeri- zation in the fringe, the tip-focused calcium gradient biases secretion towards the polar axis. The recent data showing that exocytosis of new wall material precedes and predicts the process of cell elongation provide support for the idea that the intussusception of newly secreted pectin contributes to decreases in apical wall viscosity and to cell expansion. Other prime factors will be the localization and activity of the enzyme pectin methyl-esterase, and the chelation of calcium by pectic acids. Finally, we acknowledge a role for reactive oxygen species in the control of wall viscosity.