Measurement of Ca^(2+) Flow in Cochlear Cells Using Non-Invasive Micro-Test Technique

作     者：CHEN Shi-qin YU Ning YE Sheng-nan YANG Shi-ming ZHAI Suo-qiang 

作者机构：Department of Otolaryngology Head and Neck SurgeryOto-Neurobiology CentreInstitute of OtolaryngologyChinese PLA General HospitalBeijing 100853China Department of OtolaryngologyAffiliated First HospitalFujian Medical UniversityFuzhou 350005China 

出 版 物：《Journal of Otology》 (中华耳科学杂志(英文版))

年 卷 期：2010年第5卷第2期

页      面：90-96页

学科分类：1003[医学-口腔医学] 1002[医学-临床医学] 100213[医学-耳鼻咽喉科学] 10[医学] 

基　　金：supported by NSFC grant(30600700 30772413) 

主　　题：Guinea pig outer hair cells Ca2+ ion non-invasive micro-test technique nimodipine. 

摘      要：Objective To test Calcium ion(Ca2+) flow at the head and end of outer hair cells(OHCs) in resting state and in response to Nimodipine *** Non-invasive micro-test techniques were used to study Ca2+ in isolated OHCs in adult guinea *** Four types of Ca2+ transport were identified in OHCs on basilar membrane tissue fragments:influx at the head of with efflux at the bottom(type 1):efflux at the head of OHCs with influx at the bottom(type 2);influx at the both head and bottom(type 3);and efflux at the both head and bottom(type 4).However,only type 1 and type 3 of Ca2+ ion transport were detected in the *** propose that Ca2+ ion transport exists in adult guinea pig cochlear OHCs in resting state and is ***2 + flow in OHC can be inhibited by Nimodipine in resting state.