Correlation of in vivo tumor response and singlet oxygen luminescence detection in mTHPC-mediated photodynamic therapy

作     者：Brian C.Wilson Michael S.Patterson Buhong Li Mark T.Jarvi 

作者机构：Department of Medical Biophysics University of Toronto/University Health Network TorontoOntario M5G 1L7Canada Juravinski Cancer Centre and McMaster University HamiltonOntario L8V 5C2Canada MOE Key Laboratory of OptoElectronic Science and Technology for Medicine Fujian Provincial Key Laboratory for Photonics Technology Fujian Normal UniversityFuzhouFujian 350007P.R.China 

出 版 物：《Journal of Innovative Optical Health Sciences》 (创新光学健康科学杂志（英文）)

年 卷 期：2015年第8卷第1期

页      面：106-112页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0808[工学-电气工程] 1002[医学-临床医学] 0809[工学-电子科学与技术（可授工学、理学学位）] 0805[工学-材料科学与工程（可授工学、理学学位）] 100214[医学-肿瘤学] 0836[工学-生物工程] 0702[理学-物理学] 10[医学] 

基　　金：supported by the Canadian Cancer Society Research Institute (014245) the Fujian Provincial Natural Science Foundation (2011J06022) 

主　　题：Photodynamice therapy singlet axygen lumninescence dosinetry bioluminescence 

摘      要：Excited-state singlet axygen(^(1)O_(2)),generated during photodynamic therapy(PDT),is believed to be the primary cytotoxic agent with a number of clinically approved *** relative concentration in cells or tissues can be measured directly through its near-infrared(NIR)luminescence emission,which has correlated well with in vitro cell and in triro normal skin treatment ***,its correlation with the response of tumor tiss1e in vito is examined,using the photosensitizer meso-tetralydroxyphenylchlorin(mTHPC)in an animal model comprising luciferase-and green fluorescent protein(GFP)-transduced gliosarcoma grown in a dorsal window *** change in the bioluminescence signal,imaged pre-treatment and at 2,5 and 9 d post treatment,was used as a quantitative measure of the tumor response,which was classified in individual tumors asnon,moderateandstrongin order to reduce the variance in the *** the bioluminescence-based response vs the^(1)O_(2)counts demonstrated clear correlation,indicating tha^(1)O_(2)luminescence provides a valid do-simetric technique for PDT in tumor tissue.