Germline silencing of UASt depends on the piRNA pathway

作     者：Yi-Chun Huang Henry Moreno Sarayu Row Dongyu Jia Wu-Min Deng 

作者机构：Department of Biological ScienceFlorida State UniversityTallahasseeFL 32306-4295USA Department of BiologyGeorgia Southern UniversityStatesboro30460-8042USA. 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2018年第45卷第5期

页      面：273-276页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：supported by NIH Grant R01GM072562 and NSF Grant IOS1557904 

主　　题：RFP UAS Germline silencing of UASt depends on the piRNA pathway AGO UTR 

摘      要：The success of the fruit fly Drosophila melanogaster as a model organism is heavily attributed to the expansive range and multitude of genetic and molecular tools available to modify gene expression at will. The GaI4/UAS binary system is one of the most important and widely used genetic tools in Drosophila designed for targeted gene expression （Brand and Perrimon, 1993）, which allows ectopic expression of any gene （or transgene） in specific tissues, independent of their native regulators. However, a drawback of the original UASt-transgene is its silence in germline cells--when the UASt-RFPhis transgene was driven by a ubiquitously expressed 6al4 under the ActinSC promoter （act-Gal4）, RFP expression was only detected in the somatic follicle cells, but not in the germline nurse cells or the oocyte （Fig. 1A）.