Synergistic effect and different toxicities of adjuvant components of Realgar–Indigo Naturalis formula

作     者：Huan-Hua Xu Zeng-Chun Ma Qiao-Li Shi Shi-Han Yang La Jiang Xiang-Mei Chen Yue Gao 

作者机构：Guangdong Pharmaceutical UniversityGuangzhou 510006China Institute of Radiation MedicineAcademy of Military Medical SciencesBeijing 100850China State Key Laboratory of Drug MetabolismHematological PharmacologyBeijing Institute of Transfusion MedicineBeijing 100850China Department of NephrologyChinese PLA General HospitalChinese PLA Institute of NephrologyState Key Laboratory of Kidney DiseasesNational Clinical Research Center for Kidney DiseasesBeijing 100850China 

出 版 物：《Chinese Herbal Medicines》 (中草药（英文版）)

年 卷 期：2018年第10卷第2期

页      面：137-144页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：financially supported by a Grant from the National Ministry of Science and Technology(no.2015ZX09501004) 

主　　题：acute toxicity arsenic valences realgar sub chronic toxicity toxic potentials 

摘      要：Objective：Realgar–Indigo Naturalis formula（RIF）is a well-known arsenic-containing preparation that is used to treat acute promyelocytic leukemia（APL）in *** recent multicenter clinical trials,complete remission rates in APL patients have ranged from 96.08%to 100%.RIF has a satisfactory therapeutic effect,but its safety is a widespread concern,since the preparation contains arsenic,a wide-ranging and naturally occurring *** this study,in order to determine the toxic potential of RIF,acute toxicity and sub chronic toxicity assays were performed to evaluate the toxic potential of Realgar and the adjuvant components of RIF in addition to Realgar’s synergy with these adjuvant ***：KM mice and Wistar rats were selected for these *** evaluate acute toxicity,the toxic effects of a single dose of a gradient of concentrations of Realgar were firstly ***,the toxic effects of combinations of gradient doses of Realgar and fixed doses of Indigo naturalis and Salvia miltiorrhiza were ***：The results showed that when Realgar was used alone,the2756.73 mg/kg（equivalent to 23.6 mg/kg）.However,theto 936.90 mg/kg when Realgar was used with *** contrast,theto 7538.86 mg/kg when Realgar was used with ***,*** strengthened the toxicity of Realgar,whereas *** displayed the opposite *** sub chronic toxicity assessment results revealed a trend that was consistent with acute *** in the levels of different valence states of arsenic were also taken into *** test results of the effects of in vitro combinations of Realgar and adjuvant components on soluble arsenic dissolution showed that *** increased the level of soluble arsenic in Realgar extracts and *** suspensions when the Realgar/*** ratio was 2,1.5,and ***,*** did not affect ***：Based on the collective experimental results presented here,it can be conclu