Cloning and Bioinformation Analysis of Carbonic Anhydrase Gene FsCA1 of Tibet Wild Buckwheat

作     者：Weihai HOU Jianlin WANG Danba Hudan 

作者机构：Key Laboratory of Plateau Crop Molecular BreedingTibet Agricultural and Animal Husbandry College 

出 版 物：《Asian Agricultural Research》 (亚洲农业研究（英文）)

年 卷 期：2017年第9卷第11期

页      面：54-57,65页

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：Supported by Project of National Natural Science Foundation(31360300&31560362) Key Project of the Tibet Autonomous Region(XZXTCX-2016) 

主　　题：Tibet buckwheat Carbonic anhydrase(CA) FsCA1 Three-dimensional structure prediction Bioinformatics 

摘      要：A carbonic anhydrase( CA) transcript was obtained from the Contig library according to the published sequencing information of the buckwheat transcripts. The full length of the CA gene was amplified by reverse transcription PCR( RT-PCR). The bioinformatics analysis showed that the full length of Fs CA1 gene was 1233 bp and open reading frame was 978 bp,and encoding 325 amino acids. The molecular weight was 35. 11 ku and the isoelectric point was 7. 59; there were 9 α helices,6 β folds,many randon coil and extension chain,containing one signal peptide and one transmembrane region,having a 2 amino acid conserved domains with typical beta-type carbonic anhydrase. Subcellular localization showed that the protein is most likely to appear in the chloroplast. The three-dimensional structure model of Fs CA1 was built by homologous modeling method,indicating that the homo-octamer of buckwheat CA and pea CA could match well,so it can be inferred that buckwheat CA is also homo-octamer. Real-time quantitative PCR was used to detect the expression of Fs CA1 in different organs of *** results showed that Fs CA1 had the highest expression level in leaves,then in the stems,and the lowest in roots.