Characterization ofanovel deep-seamicrobial esterase EstC 10 and its use in the generation o f(R)-methyl 2-chloropropionate

作     者：GONG Yanhui MA Sanmei WANG Yongfei XU Yongkai SUN Aijun ZHANG Yun HU Yunfeng 公颜慧;马三梅;王永飞;许永楷;孙爱君;张云;胡云峰

作者机构：Key Laboratory of Tropical Marine Bio-Resources and Ecology South China Sea Institute of Oceanology Chinese Academy of Sciences Guangzhou 510301 China Guangdong Key Laboratory of Marine Materia Mediea South China Sea Institute of Oceanology Chinese Academy of Sciences Guangzhou 510301 China Department ofBiotechnology Jinan University Guangzhou 510632 China Affiliated Hospital of Shandong University of Traditional Chinese Medicine Jinan 250014 China South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center Guangzhou 510275 China 

出 版 物：《Journal of Oceanology and Limnology》 (海洋湖沼学报（英文）)

年 卷 期：2018年第36卷第2期

页      面：473-482页

核心收录：

学科分类：07[理学] 

基　　金：Supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA11030404) the Guangzhou Science and Technology Plan Projects(No.201510010012) the National Natural Science Foundation of China(No.21302199) 

主　　题：biocatalysis deep-sea microorganisms novel esterase kinetic resolution (R)-methyl 2-chloropropionate 

摘      要：A novel esterase EstC10 from Bacillus sp. CX01 isolated from the deep sea of the Western Pacific Ocean and the functionalities of EstC 10 was characterized. At present, the reports about the kinetic resolution ofracemic methyl 2-chloropropionate were quite rare. So we developed deep-sea microbial esterase EstC10 as a novel biocatalyst in the kinetic resolution of racemic methyl 2-chloropropionate and generate （R）-methyl 2-chloropropionate with high enantiomeric excess （〉99%） after the optimization of process parameters such as pH, temperature, organic co-solvents, surfactants, substrate concentration and reaction time. Notably, the optimal substrate concentration （80 mmol/L） of esterase EstC10 was higher than the kinetic resolution of another esterase, Estl2-7 （50 mmoFL）. The novel microbial esterase EstC10 identified from the deep sea was a promising green biocatalyst in the generation of （R）-methyl 2-chloropropionate as well of many other valuable chiral chemicals in industry.