Pharmacological actions of miltirone in the modulation of platelet function

作     者：Zhang-yin MING Wei SONG 

作者机构：Department of Pharmacology School of Basic Medicine Tongji Medical College of Huazhong University of Science and Technology Wuhan 430030 China The Key Laboratory for DrugTarget Research and Pharmacodynamic Evaluation of Hubei Province Wuhan 430 

出 版 物：《中国药理学与毒理学杂志》 (Chinese Journal of Pharmacology and Toxicology)

年 卷 期：2018年第32卷第4期

页      面：252-253页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by National Natural Science Fund of China(81273574) Chinese Herb Key Project by Health and Family Planning Commission of Hubei Province 

主　　题：丹参 速效药 治疗方法 临床分析 

摘      要：OBJECTIVE Salvia miltiorrhiza bunge contains various active constituents,some of which have been developed as commercially available ***,some other ingredients in Salvia miltiorrhiza play great roles in anti-platelet *** aim of the present study was to investigate the effects and the underlying mechanism of miltirone,a lipophilic compound of Salvia miltiorrhiza *** The ability of miltirone to modulate platelet function was investigated by a variety of in vitro and in vivo *** aggregation and dense granule secretion induced by various agonists were measured with platelet *** retraction and spreading were imaged by digital camera and fl uorescence *** chloride-induced carotid injury model and pulmonary thromboembolism model were used to check miltirone effect in *** elucidate the mechanisms of anti-platelet activity of miltirone,flow cytometry and Western blotting were *** Miltirone(2,4,8 μmol·L^(-1)) was shown to suppress platelet aggregation,dense granule and α granule secretion in a dose-dependent ***,miltirone inhibited the clot retraction and spreading of washed *** reduced the phosphorylation of PLCγ2,PKC,Akt,GSK3β and ERK1/2 in the downstream signal pathway of collagen *** also reduced the phosphorylation of Src and FAK in the integrin αⅡbβ3 mediated outside-in signaling,while it did not suppress the phosphorylation of β*** addition,miltirone prolonged the occlusion time and reduced collagen/epinephrine induced pulmonary *** Miltirone suppresses platelet inside-out and outside-in signaling by affecting PLCγ2/PKC/ERK1/2,PI3K/Akt and Src/FAK ***,miltirone might represent a potential anti-platelet candidate for the prevention of thrombotic disorders.