The Critical Role of IL-10 in the Anti-neuroinflammatory and Anti-oxidative Effects of Rheum Tanguticum on Activated Microglia

作     者：MENG Jie NI Jun-jun WU Zhou ZHU Ai-qin QING Hong Hiroshi Nakanishi 

作者机构：Department of Aging Science and PharmacologyFaculty of Dental SciencesKyushu University OBT Research CenterFaculty of Dental SciencesKyushu University Institution of Geriatric Qinghai Provincial Hospital School of Life ScienceBeijing Institute of Technology 

出 版 物：《神经药理学报》 (Acta Neuropharmacologica)

年 卷 期：2018年第2期

页      面：26-27页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

主　　题：IL The Critical Role of IL-10 in the Anti-neuroinflammatory and Anti-oxidative Effects of Rheum Tanguticum on Activated Microglia NOS TNF 

摘      要：Objective:Rheum tanguticum Maxim. ex Balf.（Rt）,a traditional Tibetan medicine,is known to exert various bioactivities,including anti-inflammatory and anti-oxidative activities. The present study was conducted to investigate anti-inflammatory and anti-oxidative effects of Rt on activated microglia. Methods:（1）MG6 microglial cell line,primary microglia and mice brain slices culture were stimulated by chromogranin A（CGA）,an endogenous microglia activator,to induce proinflammation and oxidation. The anti-inflammatory and anti-oxidative effects of Rt were examined by q-PCR,western blotting and ELISA by checking inflammatory and oxidative molecules,including IL-1β,tumor necrosis factor-α,IL-10 and nitric oxide.（2）Investigation of the involvement of IL-10 in the anti-inflammatory effects of Rt on inflammatory responses of microglia by using neutralizing antibodies against IL-10. Pro-inflammatory molecules expression were checked by q-PCR.（3）Examination of the effects of major components of Rt(aloe-emodin,（+）-cathechin,piceatannol chrisophanol,physcion,β-sitosterol and emodin) on the IL-10 mRNA expression in MG6 cells. Results:（1）The mean mRNA expression levels of TNF-α,IL-1β and i NOS were significantly increased in MG6 cells at 24 h after stimulation with CGA. Rt significantly suppressed the mean mRNA expression levels of TNF-α,IL-1βand i NOS in GGA-stimulated MG6 cells. Furthermore,the production of IL-1β and NO metabolites in the culture medium of MG6 cells were assessed by using ELISA and NO;/NO;assay,respectively. The mean levels of IL-1β and NO;/NO;significantly increased in the culture medium of MG6 cells at 24 h after treatment with CGA. Rt also significantly decreased the mean levels of IL-1β and NO;/NO;in the culture medium of MG6 cells. The mean protein level of IL-1β significantly increased in the organotypic hippocampal slice cultures at 48 h after stimulation with CGA(10 nmol·L;). Rt significantly suppressed the mean protein level of IL-1β in CGA-stimulated o