Comparison of immunofluorescence mapping and immunohistochemistry in the auxiliary diagnosis of congenital epidermolysis bullosa

作     者：Ling Yu Su-Ying Feng Zhi-Liang Li Pei-Ying Jin Baoxi Wang 

作者机构：Department of Dermatology Henan Provincial People's Hospital (People's Hospital of Zhengzhou University) Zhengzhou Henan 450003 China. Department of Dermatology Institute of Dermatology Chinese Academy of Medical Sciences and Peking Union Medical College Nanjing Jiangsu 210042 China. Department of Dermatology Plastic Surgery Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100144 China. 

出 版 物：《International Journal of Dermatology and Venereology》 (国际皮肤性病学杂志（英文）)

年 卷 期：2018年第1卷第1期

页      面：40-43页

学科分类：10[医学] 

基　　金：This work was supported by grants from Young foundation of Peking Union Medical College (No. 3332016107)  Nature Science Foundation of Jiangsu (No. BK20160153)  Innovation Research on Critical Diseases (No. 2016ZX320014) 

主　　题：Fluorescent antibody technique Epidermolysis bullosa dystrophica Epidermolysis bullosa simplex Immunohistochemistry Diagnosis Differential Immunofluorescence mapping 

摘      要：Objective To describe the patterns of immunofluorescence mapping (IFM) in different forms of epidermolysis bullosa (EB) and classify the diagnosis of EB patients. Methods We used tissue specimens from six outpatients with congenital EB, which were made into frozen sections and paraffin sections, and stained for keratin 14 (K14), type IV collagen and type VII collagen using immunofluorescence and immunohistochemistry, simultaneously using normal skin as controls. Analysis was performed to determine which parts of the blister were labeled with the corresponding antibodies, and subtyping was performed on EB patients. Results The result showed that two cases of EBS and three cases of DEB were confirmed by IFM, while only one case of EBS and two cases of DEB were confirmed by immunohistochemistry. Conclusion IFM is an important method for subtyping EB patients, and it is superior to immunohistochemistry.