Functional Analysis of the Phosphoenolpyruvate Carboxylase on the Lipid Accumulation of Peanut(Arachis hypogaea L.) Seeds

作     者：PAN Li-juan YANG Qing-li CHI Xiao-yuan CHEN Ming-na YANG Zhen CHEN Na WANG Tong WANG Mian HE Ya-nan YU Shan-lin 

作者机构：Shandong Peanut Research Institute 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2013年第12卷第1期

页      面：36-44页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by the China Agriculture Research System (CARS-14) the National Natural Science Foundation of China (31000728,31100205) the Natural Science Fundation of Shangdong Province,China(ZR2009DQ004,ZR2011CQ036) the Promotive Research Fund for Young and Middle-Aged Scientisits of Shandong Province,China (BS2010NY023) the Qingdao Municipal Science and Technology Plan Project,China (11-2-4-9-(3)-jch,11-2-3-26-nsh) 

主　　题：peanut(Arachis hypogaea L.) phosphoenolpyruvate carboxylase PEPC activity lipid accumulation 

摘      要：Phosphoenolpyruvate carboxylase（PEPC;EC 4.***.1.31） catalyses phosphoenolpyruvate（PEP） to yield oxaloacetate,which is involved in protein *** kinase（PK;EC 2.***.1.40） catalyzes PEP to yield pyruvate,which is involved in fatty acid *** this study,five PEPC genes（AhPEPC1,AhPEPC2,AhPEPC3,AhPEPC4,and AhPEPC5） from peanut have been *** a quantitative real-time RT-PCR approach,the expression pattern of each gene was monitored during the seed development of four peanut varieties（E11,Hebeigaoyou,Naihan 1,and Huayu 26）.It was found that these five genes shared similar expression behaviors over the developmental stages of E11 with high expression levels at 30 and 40 d after pegging（DAP）;whereas these five genes showed irregular expression patterns during the seed development of *** Naihan 1 and Huayu 26,the expression levels of the five genes remained relatively high in the first *** PEPC activity was monitored during the seed development of four peanut varieties and seed oil content was also characterized during whole period of seed *** PEPC activity followed the oil accumulation pattern during the early stages of development but they showed a significantly negative correlation *** results suggested that PEPC may play an important role in lipid accumulation during the seed development of four peanut varieties tested.