Effect of Lang-Chuang-Ding Decoction(狼疮定汤)on DNA Methylation of CD70 Gene Promoter in Peripheral Blood Mononuclear Cells of Female Patients with Systemic Lupus Erythematosus

作     者：SUN Jing1 SHAO Tie-juan2 ZHANG Dong-yu2 HUANG Xue-qin2 XIE Zhi-jun2 WEN Cheng-ping2 SUN Jing;SHAO Tie-juan;ZHANG Dong-yu;HUANG Xue-qin;XIE Zhi-jun;WEN Cheng-ping

作者机构：Department of Rheumatism the Second Hospital Affiliated to Zhejiang Chinese Medical University College of Basic Medical Science Zhejiang Chinese Medical University 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2018年第24卷第5期

页      面：348-352页

核心收录：

学科分类：100506[医学-中医内科学] 1006[医学-中西医结合] 1005[医学-中医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by the National Natural Science Foundation of China(No.81373633) the Traditional Chinese Medicine Scientific Research Fund of Zhejiang Province,China(No.2016ZA095) 

主　　题：systemic lupus erythematosus Lang-Chuang-Ding Decoction Chinese medicine DNA methylation CD70 patient 

摘      要：Objective: To investigate the effect of Lang-chuang-ding Decoction(狼疮定汤, LCD) on the expression of DNA methylation of CD70 gene promoter in peripheral blood mononuclear cells(PBMCs) of females with systemic lupus erythematosus(SLE). Methods: PBMCs isolated from female patients with SLE or healthy donors were cultured and treated with LCD medicated serum or normal serum for 24 or 48 h. The m RNA expressions of CD70 gene in PBMCs were detected by reverse transcription polymerase chain reaction(PCR); the DNA methylation of the CD70 gene promoter region was detected by methylation-specific PCR. Results: After treated with medicated serum for 48 h, the m RNA expression levels of CD70 in PBMCs of SLE patients were significantly higher than those of healthy donors(P0.05); the DNA methylation levels of CD70 promoter region in PBMCs of SLE patients treated with medicated serum for 48 h were significantly higher than those treated with fetal bovine serum(P0.01). Conclusion: LCD could inhibit CD70 gene expression in PBMCs of SLE patients by promoting the DNA methylation of CD70 gene promoter.