Chemical Diversity Investigation of Hepatotoxic Pyrrolizidine Alkaloids in Qianliguang(Senecio scandens)and Related Species by UHPLC-QTOF-MS

作     者：Lin Zhu Na Li Jian-Qing Ruan Peter P.Fu Zhong-Zhen Zhao Ge Lin 

作者机构：School of Biomedical Sciences Faculty of Medicine The Chinese University of Hong Kong State Key Lab for Quality Research in Chinese Medicines Macao Institute for Applied Research in Medicine and Health Macao University of Science and Technology National Center for Toxicological Research School of Chinese Medicine Hong Kong Baptist University 

出 版 物：《World Journal of Traditional Chinese Medicine》 (世界中医药杂志（英文）)

年 卷 期：2015年第1卷第2期

页      面：1-11页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Research Grant Council of Hong Kong(GRF Grants no.471310 and 469712) CUHK Direct Grant(2041744) 

主　　题：Qianliguang(Senecio scandens) Pyrrolizidine alkaloids UHPLC-QTOF-MS Qualitation Quantification 

摘      要：Objective: Qianliguang(Senecio scandens) is a common Chinese medicinal herb. Qianliguang-containing herbal proprietary products are registered as over-the-counter remedies in China and exported to Western countries. The presence of hepatotoxic pyrrolizidine alkaloids(PAs)has raised concerns about the safety of using Qianliguang and its products. The present study aims at investigation of different types of PAs present in Qianliguang collected from representative locations in ***: In this study, a simple but specific UHPLC-QTOF-MS method for the determination of toxic PAs was developed, based on the characteristic fragment ions specific to different types of PAs. It was successfully applied for the identification and distinguishing of PAs present in Qianliguang and related Senecio species growing in different locations of ***: Significant diversity of the PA types and quantities were revealed among the samples tested. The estimated total amounts of toxic PAs in three of the samples exceed the toxic limits of PA intake restricted by WHO, demonstrating the timely and highly demand for regulating both types and quantities of PAs present in ***: This study provides the methodology for simultaneous identification and quantification of PAs present in herbs without requiring corresponding standards, which could be further used for more systematic investigations of the PA distribution in Qianliguang and other PA-containing herbs.