Enzymatic treatment of estrogens and estrogen glucuronide

作     者：Takaaki Tanaka Toshiyuki Tamura Yuuichi Ishizaki Akito Kawasaki Tomokazu Kawase Masahiro Teraguchi Masayuki Taniguchi 

作者机构：Department of Materials Science and TechnologyNiigata University Venture Business LaboratoryNiigata University Department of Chemistry and Chemical EngineeringNiigata University 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2009年第21卷第6期

页      面：731-735页

核心收录：

学科分类：083001[工学-环境科学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 08[工学] 

基　　金：supported by the grant from Sasaki Environment Technology Foundation to T.Tanaka 

主　　题：enzymatic treatment laccase estrone estradiol ethynylestradiol glucuronidase glucuronide 

摘      要：Natural and synthetic estrogens from sewage treatment systems are suspected to influence the reproductive health of the animals in the rivers. In this article, we investigated the enzymatic treatment of three estrogens （estrone, 17β-estradiol, and 17α-ethynyletstradiol） by a fungal laccase which oxidize phenolic compounds with dissolved oxygen. The elimination of the estrogenic activities by enzymatic oxidation was demonstrated by medaka vitellogenin assay. In addition, we developed an enzymatic treatment system comprised of β-D-glucuronidase and the laccase for 17β-estradiol 3-（β-D-glucuronide） degradation. The two enzymes eliminated 17β-estradiol 3-（β- D-glucuronide） and the intermediate, 17β-estradiol, efficiently.